Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Zhijuan Wang; Juan Zhang; Peng Chen; Xiaozhu Zhou; Yanli Yang; Shixin Wu; Li Niu; Yu Han; Lianhui Wang; Peng Chen; Freddy Boey; Qichun Zhang; Bo Gunnar Liedberg; Hua Zhang

doi:10.1016/j.bios.2011.03.002

Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Zhijuan Wang, Juan Zhang, Peng Chen, Xiaozhu Zhou, Yanli Yang, Shixin Wu, Li Niu, Yu Han, Lianhui Wang, Peng Chen, Freddy Boey, Qichun Zhang, Bo Gunnar Liedberg, Hua Zhang

Research output: Contribution to journal › Article › peer-review

198 Scopus citations

Abstract

Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity. © 2011 Elsevier B.V.

Original language	English (US)
Pages (from-to)	3881-3886
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	26
Issue number	9
DOIs	https://doi.org/10.1016/j.bios.2011.03.002
State	Published - May 2011

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the AcRF Tier 2 (ARC 10/10, no. MOE2010-T2-1-060) from MOE, POC (S08/1-82563404) from EDB, CRP (NRF-CRP2-2007-01) from NRF, an A*STAR SERC Grant (no. 092 101 0064) from A*STAR,New Initiative Fund FY 2010 (M58120031) from NTU, and the Centre for Biomimetic Sensor Science at NTU in Singapore. P.C. thanks the financial support from A*STAR SERC (grant no. 072 101 0020) in Singapore. B.L. also acknowledges support from the Swedish Research Council and the NanoSence program the Foundation for Strategic Research.

ASJC Scopus subject areas

Biomedical Engineering
Electrochemistry
Biotechnology
Biophysics

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10.1016/j.bios.2011.03.002

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Wang, Z., Zhang, J., Chen, P., Zhou, X., Yang, Y., Wu, S., Niu, L., Han, Y., Wang, L., Chen, P., Boey, F., Zhang, Q., Liedberg, B. G., & Zhang, H. (2011). Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes. Biosensors and Bioelectronics, 26(9), 3881-3886. https://doi.org/10.1016/j.bios.2011.03.002

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title = "Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes",

abstract = "Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity. {\textcopyright} 2011 Elsevier B.V.",

author = "Zhijuan Wang and Juan Zhang and Peng Chen and Xiaozhu Zhou and Yanli Yang and Shixin Wu and Li Niu and Yu Han and Lianhui Wang and Peng Chen and Freddy Boey and Qichun Zhang and Liedberg, {Bo Gunnar} and Hua Zhang",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the AcRF Tier 2 (ARC 10/10, no. MOE2010-T2-1-060) from MOE, POC (S08/1-82563404) from EDB, CRP (NRF-CRP2-2007-01) from NRF, an A*STAR SERC Grant (no. 092 101 0064) from A*STAR,New Initiative Fund FY 2010 (M58120031) from NTU, and the Centre for Biomimetic Sensor Science at NTU in Singapore. P.C. thanks the financial support from A*STAR SERC (grant no. 072 101 0020) in Singapore. B.L. also acknowledges support from the Swedish Research Council and the NanoSence program the Foundation for Strategic Research.",

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Wang, Z, Zhang, J, Chen, P, Zhou, X, Yang, Y, Wu, S, Niu, L, Han, Y, Wang, L, Chen, P, Boey, F, Zhang, Q, Liedberg, BG & Zhang, H 2011, 'Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes', Biosensors and Bioelectronics, vol. 26, no. 9, pp. 3881-3886. https://doi.org/10.1016/j.bios.2011.03.002

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AU - Wang, Lianhui

AU - Chen, Peng

AU - Boey, Freddy

AU - Zhang, Qichun

AU - Liedberg, Bo Gunnar

AU - Zhang, Hua

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the AcRF Tier 2 (ARC 10/10, no. MOE2010-T2-1-060) from MOE, POC (S08/1-82563404) from EDB, CRP (NRF-CRP2-2007-01) from NRF, an A*STAR SERC Grant (no. 092 101 0064) from A*STAR,New Initiative Fund FY 2010 (M58120031) from NTU, and the Centre for Biomimetic Sensor Science at NTU in Singapore. P.C. thanks the financial support from A*STAR SERC (grant no. 072 101 0020) in Singapore. B.L. also acknowledges support from the Swedish Research Council and the NanoSence program the Foundation for Strategic Research.

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N2 - Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity. © 2011 Elsevier B.V.

AB - Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity. © 2011 Elsevier B.V.

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ER -

Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Abstract

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