Label-free detection of DNA hybridization using transistors based on CVD grown graphene

Tzu Yin Chen; Phan Thi Kim Loan; Chang Lung Hsu; Yi Hsien Lee; Jacob Tse-Wei Wang; Kung Hwa Wei; Cheng Te Lin; Lain Jong Li

doi:10.1016/j.bios.2012.07.059

Label-free detection of DNA hybridization using transistors based on CVD grown graphene

Tzu Yin Chen, Phan Thi Kim Loan, Chang Lung Hsu, Yi Hsien Lee, Jacob Tse-Wei Wang, Kung Hwa Wei, Cheng Te Lin^*, Lain Jong Li

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

196 Scopus citations

Abstract

The high transconductance and low noise of graphene-based field-effect transistors based on large-area monolayer graphene produced by chemical vapor deposition are used for label-free electrical detection of DNA hybridization. The gate materials, buffer concentration and surface condition of graphene have been optimized to achieve the DNA detection sensitivity as low as 1pM (10^-12M), which is more sensitive than the existing report based on few-layer graphene. The graphene films obtained using conventional PMMA-assisted transfer technique exhibits PMMA residues, which degrade the sensing performance of graphene. We have demonstrated that the sensing performance of the graphene samples prepared by gold-transfer is largely enhanced (by 125%).

Original language	English (US)
Pages (from-to)	103-109
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/j.bios.2012.07.059
State	Published - Mar 15 2013
Externally published	Yes

Keywords

CVD graphene
DNA sensing
Gold-transfer method
Label-free detection
Liquid-gated transistors

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2012.07.059

Cite this

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title = "Label-free detection of DNA hybridization using transistors based on CVD grown graphene",

abstract = "The high transconductance and low noise of graphene-based field-effect transistors based on large-area monolayer graphene produced by chemical vapor deposition are used for label-free electrical detection of DNA hybridization. The gate materials, buffer concentration and surface condition of graphene have been optimized to achieve the DNA detection sensitivity as low as 1pM (10-12M), which is more sensitive than the existing report based on few-layer graphene. The graphene films obtained using conventional PMMA-assisted transfer technique exhibits PMMA residues, which degrade the sensing performance of graphene. We have demonstrated that the sensing performance of the graphene samples prepared by gold-transfer is largely enhanced (by 125%).",

keywords = "CVD graphene, DNA sensing, Gold-transfer method, Label-free detection, Liquid-gated transistors",

author = "Chen, {Tzu Yin} and Loan, {Phan Thi Kim} and Hsu, {Chang Lung} and Lee, {Yi Hsien} and {Tse-Wei Wang}, Jacob and Wei, {Kung Hwa} and Lin, {Cheng Te} and Li, {Lain Jong}",

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doi = "10.1016/j.bios.2012.07.059",

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AU - Chen, Tzu Yin

AU - Loan, Phan Thi Kim

AU - Hsu, Chang Lung

AU - Lee, Yi Hsien

AU - Tse-Wei Wang, Jacob

AU - Wei, Kung Hwa

AU - Lin, Cheng Te

AU - Li, Lain Jong

PY - 2013/3/15

Y1 - 2013/3/15

N2 - The high transconductance and low noise of graphene-based field-effect transistors based on large-area monolayer graphene produced by chemical vapor deposition are used for label-free electrical detection of DNA hybridization. The gate materials, buffer concentration and surface condition of graphene have been optimized to achieve the DNA detection sensitivity as low as 1pM (10-12M), which is more sensitive than the existing report based on few-layer graphene. The graphene films obtained using conventional PMMA-assisted transfer technique exhibits PMMA residues, which degrade the sensing performance of graphene. We have demonstrated that the sensing performance of the graphene samples prepared by gold-transfer is largely enhanced (by 125%).

AB - The high transconductance and low noise of graphene-based field-effect transistors based on large-area monolayer graphene produced by chemical vapor deposition are used for label-free electrical detection of DNA hybridization. The gate materials, buffer concentration and surface condition of graphene have been optimized to achieve the DNA detection sensitivity as low as 1pM (10-12M), which is more sensitive than the existing report based on few-layer graphene. The graphene films obtained using conventional PMMA-assisted transfer technique exhibits PMMA residues, which degrade the sensing performance of graphene. We have demonstrated that the sensing performance of the graphene samples prepared by gold-transfer is largely enhanced (by 125%).

KW - CVD graphene

KW - DNA sensing

KW - Gold-transfer method

KW - Label-free detection

KW - Liquid-gated transistors

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JO - Biosensors and Bioelectronics

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Label-free detection of DNA hybridization using transistors based on CVD grown graphene

Abstract

Keywords

ASJC Scopus subject areas

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