Label-free detection of alanine aminotransferase using a graphene field-effect biosensor

Jim Long Her, Tung Ming Pan*, Wan Ying Lin, Kai Sheng Wang, Lain Jong Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


In this paper, we developed a low operation voltage and a single reaction step of a graphene field-effect transistor (GFET) biosensor device to detect alanine aminotransferase (ALT). The device is fabricated using large-area graphene thin films by means of chemical vapor deposition. The GFET device operated at 0.1 V drain voltage and -0.1 to 0.3 V sweep gate voltage exhibited a high pH sensitivity of 23.12 mV/pH, a low hysteresis voltage of 1.2 mV, and a small drift rate of 4.74 mV/h. In this research, the hybrid configuration of l-alanine and α-ketoglutarate immobilized on the graphene membrane was proved to be able to detect ALT with good linearity (R2 = 0.99) in the ALT concentration range of 10-100 U/L.

Original languageEnglish (US)
Pages (from-to)396-400
Number of pages5
JournalSensors and Actuators, B: Chemical
StatePublished - 2013
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the National Science Council, Taiwan, Republic of China , under contract no. NSC-97-2221-E-182-050-MY3 .


  • Alanine aminotransferase (ALT)
  • Graphene field-effect transistor (GFET)
  • l-Alanine
  • α-Ketoglutarate

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Metals and Alloys
  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electrical and Electronic Engineering


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