Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin

Felix Quitterer; Anja List; Wolfgang Eisenreich; Adelbert Bacher; Michael Groll

doi:10.1002/ange.201106765

Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin

Felix Quitterer, Anja List, Wolfgang Eisenreich, Adelbert Bacher, Michael Groll

Research output: Contribution to journal › Article › peer-review

Abstract

Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation–recombination mechanism via a glycyl radical intermediate.

Original language	English (US)
Pages (from-to)	1367-1370
Number of pages	4
Journal	Angewandte Chemie
Volume	124
Issue number	6
DOIs	https://doi.org/10.1002/ange.201106765
State	Published - Nov 16 2011
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): FIC/2010/07
Acknowledgements: Wir danken Sophie Vieweg fur ihre experimentelle Unterstutzung und den Mitarbeitern der PXI des Paul Scherrer Instituts, Swiss Light Source (Villigen, Schweiz) fur ihre Hilfe beim Messen der Datensutze. Diese Arbeit wurde von der Hans-Fischer-Gesellschaft und der King Abdullah University of Science and Technology (Award No. FIC/2010/07) gefçrdert.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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title = "Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin",

abstract = "Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation–recombination mechanism via a glycyl radical intermediate.",

author = "Felix Quitterer and Anja List and Wolfgang Eisenreich and Adelbert Bacher and Michael Groll",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): FIC/2010/07 Acknowledgements: Wir danken Sophie Vieweg fur ihre experimentelle Unterstutzung und den Mitarbeitern der PXI des Paul Scherrer Instituts, Swiss Light Source (Villigen, Schweiz) fur ihre Hilfe beim Messen der Datensutze. Diese Arbeit wurde von der Hans-Fischer-Gesellschaft und der King Abdullah University of Science and Technology (Award No. FIC/2010/07) gef{\c c}rdert. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2011",

month = nov,

day = "16",

doi = "10.1002/ange.201106765",

language = "English (US)",

volume = "124",

pages = "1367--1370",

journal = "Angewandte Chemie",

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AU - Quitterer, Felix

AU - List, Anja

AU - Eisenreich, Wolfgang

AU - Bacher, Adelbert

AU - Groll, Michael

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): FIC/2010/07 Acknowledgements: Wir danken Sophie Vieweg fur ihre experimentelle Unterstutzung und den Mitarbeitern der PXI des Paul Scherrer Instituts, Swiss Light Source (Villigen, Schweiz) fur ihre Hilfe beim Messen der Datensutze. Diese Arbeit wurde von der Hans-Fischer-Gesellschaft und der King Abdullah University of Science and Technology (Award No. FIC/2010/07) gefçrdert. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2011/11/16

Y1 - 2011/11/16

N2 - Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation–recombination mechanism via a glycyl radical intermediate.

AB - Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation–recombination mechanism via a glycyl radical intermediate.

UR - http://hdl.handle.net/10754/598689

UR - http://doi.wiley.com/10.1002/ange.201106765

U2 - 10.1002/ange.201106765

DO - 10.1002/ange.201106765

M3 - Article

SN - 0044-8249

VL - 124

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EP - 1370

JO - Angewandte Chemie

JF - Angewandte Chemie

IS - 6

ER -

Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin

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