Isolation of discrete nanoparticle-DNA conjugates for plasmonic applications

Shelley A. Claridge; Huiyang W. Liang; S. Roger Basu; Jean M.J. Fréchet; A. Paul Alivisatos

doi:10.1021/nl0802032

Isolation of discrete nanoparticle-DNA conjugates for plasmonic applications

Shelley A. Claridge, Huiyang W. Liang, S. Roger Basu, Jean M.J. Fréchet, A. Paul Alivisatos

Physical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

154 Scopus citations

Abstract

Discrete DNA gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (≥20 nm) are difficult to purify by other means and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.

Original language	English (US)
Pages (from-to)	1202-1206
Number of pages	5
Journal	Nano Letters
Volume	8
Issue number	4
DOIs	https://doi.org/10.1021/nl0802032
State	Published - Apr 1 2008

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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abstract = "Discrete DNA gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (≥20 nm) are difficult to purify by other means and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.",

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AU - Basu, S. Roger

AU - Fréchet, Jean M.J.

AU - Alivisatos, A. Paul

PY - 2008/4/1

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AB - Discrete DNA gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (≥20 nm) are difficult to purify by other means and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.

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Isolation of discrete nanoparticle-DNA conjugates for plasmonic applications

Abstract

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