Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Stefania De Vitis; Giuseppina Matarise; Francesca Pardeo; Rossella Catalano; Natalia Maria Malara; Valentina Trunzo; Rossana Tallerico; Francesco T. Gentile; Patrizio Candeloro; Maria Laura Coluccio; Alessandro S. Massaro; Giuseppe Viglietto; Ennio Carbone; Jörg Peter Kutter; Gerardo Perozziello; Enzo M. Di Fabrizio

doi:10.1016/j.mee.2014.04.013

Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Stefania De Vitis, Giuseppina Matarise, Francesca Pardeo, Rossella Catalano, Natalia Maria Malara, Valentina Trunzo, Rossana Tallerico, Francesco T. Gentile, Patrizio Candeloro, Maria Laura Coluccio, Alessandro S. Massaro, Giuseppe Viglietto, Ennio Carbone, Jörg Peter Kutter, Gerardo Perozziello, Enzo M. Di Fabrizio

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs. © 2014 Elsevier B.V. All rights reserved.

Original language	English (US)
Pages (from-to)	76-80
Number of pages	5
Journal	Microelectronic Engineering
Volume	124
DOIs	https://doi.org/10.1016/j.mee.2014.04.013
State	Published - Jul 2014

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (code: PON01_02782), the European project EUROMBR (grant agreement n.608104), the project for Young Researchers financed from the Italian Ministry of Health (CUP J65C13001350001, project n. GR-2010-2311677) and the project FIRB "ReteNazionale di Ricerca sulle Nanoscienze ItalNanoNet" (code: RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro.

ASJC Scopus subject areas

Surfaces, Coatings and Films
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Electrical and Electronic Engineering
Condensed Matter Physics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mee.2014.04.013

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De Vitis, S., Matarise, G., Pardeo, F., Catalano, R., Malara, N. M., Trunzo, V., Tallerico, R., Gentile, F. T., Candeloro, P., Coluccio, M. L., Massaro, A. S., Viglietto, G., Carbone, E., Kutter, J. P., Perozziello, G., & Di Fabrizio, E. M. (2014). Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols. Microelectronic Engineering, 124, 76-80. https://doi.org/10.1016/j.mee.2014.04.013

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abstract = "The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using {"}in situ{"} microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs. {\textcopyright} 2014 Elsevier B.V. All rights reserved.",

author = "{De Vitis}, Stefania and Giuseppina Matarise and Francesca Pardeo and Rossella Catalano and Malara, {Natalia Maria} and Valentina Trunzo and Rossana Tallerico and Gentile, {Francesco T.} and Patrizio Candeloro and Coluccio, {Maria Laura} and Massaro, {Alessandro S.} and Giuseppe Viglietto and Ennio Carbone and Kutter, {J{\"o}rg Peter} and Gerardo Perozziello and {Di Fabrizio}, {Enzo M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the project PON {"}Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti{"} (code: PON01_02782), the European project EUROMBR (grant agreement n.608104), the project for Young Researchers financed from the Italian Ministry of Health (CUP J65C13001350001, project n. GR-2010-2311677) and the project FIRB {"}ReteNazionale di Ricerca sulle Nanoscienze ItalNanoNet{"} (code: RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro.",

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De Vitis, S, Matarise, G, Pardeo, F, Catalano, R, Malara, NM, Trunzo, V, Tallerico, R, Gentile, FT, Candeloro, P, Coluccio, ML, Massaro, AS, Viglietto, G, Carbone, E, Kutter, JP, Perozziello, G & Di Fabrizio, EM 2014, 'Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols', Microelectronic Engineering, vol. 124, pp. 76-80. https://doi.org/10.1016/j.mee.2014.04.013

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AU - De Vitis, Stefania

AU - Matarise, Giuseppina

AU - Pardeo, Francesca

AU - Catalano, Rossella

AU - Malara, Natalia Maria

AU - Trunzo, Valentina

AU - Tallerico, Rossana

AU - Gentile, Francesco T.

AU - Candeloro, Patrizio

AU - Coluccio, Maria Laura

AU - Massaro, Alessandro S.

AU - Viglietto, Giuseppe

AU - Carbone, Ennio

AU - Kutter, Jörg Peter

AU - Perozziello, Gerardo

AU - Di Fabrizio, Enzo M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (code: PON01_02782), the European project EUROMBR (grant agreement n.608104), the project for Young Researchers financed from the Italian Ministry of Health (CUP J65C13001350001, project n. GR-2010-2311677) and the project FIRB "ReteNazionale di Ricerca sulle Nanoscienze ItalNanoNet" (code: RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro.

PY - 2014/7

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N2 - The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs. © 2014 Elsevier B.V. All rights reserved.

AB - The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs. © 2014 Elsevier B.V. All rights reserved.

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M3 - Article

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JO - Microelectronic Engineering

JF - Microelectronic Engineering

ER -

Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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