Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.

Melike Balk; Hans G H J Heilig; Miriam H A van Eekert; Alfons J M Stams; Irene C Rijpstra; Jaap S Sinninghe-Damsté; Willem M de Vos; Servé W M Kengen

doi:10.1007/s00792-009-0276-9

Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.

Melike Balk, Hans G H J Heilig, Miriam H A van Eekert, Alfons J M Stams, Irene C Rijpstra, Jaap S Sinninghe-Damsté, Willem M de Vos, Servé W M Kengen

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Abstract

A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayaş, Turkey. The cells were straight to curved rods, 0.4-0.6 microm in diameter and 3.5-10 microm in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.

Original language	English (US)
Pages (from-to)	885-894
Number of pages	10
Journal	Extremophiles
Volume	13
Issue number	6
DOIs	https://doi.org/10.1007/s00792-009-0276-9
State	Published - Aug 23 2009
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): KUK-C1-017-12
Acknowledgements: This work was supported by the INTAS and Darwin Centre for Biogeology of Netherlands Organization for Scientific Research (NWO) and was also partly supported by Award No KUK-C1-017-12, made by King Abdullah University of Science and Technology (KAUST).
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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10.1007/s00792-009-0276-9

https://repository.kaust.edu.sa/bitstream/10754/596843/1/PMC2767516.pdf

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Balk, M., Heilig, H. G. H. J., van Eekert, M. H. A., Stams, A. J. M., Rijpstra, I. C., Sinninghe-Damsté, J. S., de Vos, W. M., & Kengen, S. W. M. (2009). Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey. Extremophiles, 13(6), 885-894. https://doi.org/10.1007/s00792-009-0276-9

@article{27ff1cb016c640569eb7ed41fa455420,

title = "Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.",

abstract = "A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Aya{\c s}, Turkey. The cells were straight to curved rods, 0.4-0.6 microm in diameter and 3.5-10 microm in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.",

author = "Melike Balk and Heilig, {Hans G H J} and {van Eekert}, {Miriam H A} and Stams, {Alfons J M} and Rijpstra, {Irene C} and Sinninghe-Damst{\'e}, {Jaap S} and {de Vos}, {Willem M} and Kengen, {Serv{\'e} W M}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-C1-017-12 Acknowledgements: This work was supported by the INTAS and Darwin Centre for Biogeology of Netherlands Organization for Scientific Research (NWO) and was also partly supported by Award No KUK-C1-017-12, made by King Abdullah University of Science and Technology (KAUST). This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2009",

month = aug,

day = "23",

doi = "10.1007/s00792-009-0276-9",

language = "English (US)",

volume = "13",

pages = "885--894",

journal = "Extremophiles",

issn = "1431-0651",

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Balk, M, Heilig, HGHJ, van Eekert, MHA, Stams, AJM, Rijpstra, IC, Sinninghe-Damsté, JS, de Vos, WM & Kengen, SWM 2009, 'Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.', Extremophiles, vol. 13, no. 6, pp. 885-894. https://doi.org/10.1007/s00792-009-0276-9

TY - JOUR

T1 - Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.

AU - Balk, Melike

AU - Heilig, Hans G H J

AU - van Eekert, Miriam H A

AU - Stams, Alfons J M

AU - Rijpstra, Irene C

AU - Sinninghe-Damsté, Jaap S

AU - de Vos, Willem M

AU - Kengen, Servé W M

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-C1-017-12 Acknowledgements: This work was supported by the INTAS and Darwin Centre for Biogeology of Netherlands Organization for Scientific Research (NWO) and was also partly supported by Award No KUK-C1-017-12, made by King Abdullah University of Science and Technology (KAUST). This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2009/8/23

Y1 - 2009/8/23

N2 - A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayaş, Turkey. The cells were straight to curved rods, 0.4-0.6 microm in diameter and 3.5-10 microm in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.

AB - A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayaş, Turkey. The cells were straight to curved rods, 0.4-0.6 microm in diameter and 3.5-10 microm in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.

UR - http://hdl.handle.net/10754/596843

UR - http://link.springer.com/10.1007/s00792-009-0276-9

UR - http://www.scopus.com/inward/record.url?scp=70350699218&partnerID=8YFLogxK

U2 - 10.1007/s00792-009-0276-9

DO - 10.1007/s00792-009-0276-9

M3 - Article

C2 - 19701714

SN - 1431-0651

VL - 13

SP - 885

EP - 894

JO - Extremophiles

JF - Extremophiles

IS - 6

ER -

Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.

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