Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool

Yasmine M. Mohamed; Mohamed A. Ghazy; Ahmed Sayed; Amged Ouf; Hamza El-Dorry; Rania Siam

doi:10.1038/srep03358

Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool

Yasmine M. Mohamed, Mohamed A. Ghazy, Ahmed Sayed, Amged Ouf, Hamza El-Dorry, Rania Siam

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

The Red Sea Atlantis II brine pool is an extreme environment that displays multiple harsh conditions such as high temperature, high salinity and high concentrations of multiple, toxic heavy metals. The survival of microbes in such an environment by utilizing resistant enzymes makes them an excellent source of extremophilic enzymes. We constructed a fosmid metagenomic library using DNA isolated from the deepest and most secluded layer of this pool. We report the isolation and biochemical characterization of an unusual esterase: EstATII. EstATII is thermophilic (optimum temperature, 65 C), halotolerant (maintains its activity in up to 4.5â€...M NaCl) and maintains at least 60% of its activity in the presence of a wide spectrum of heavy metals. The combination of biochemical characteristics of the Red Sea Atlantis II brine pool esterase, i.e., halotolerance, thermophilicity and resistance to heavy metals, makes it a potentially useful biocatalyst.

Original language	English (US)
Journal	Scientific Reports
Volume	3
Issue number	1
DOIs	https://doi.org/10.1038/srep03358
State	Published - Nov 28 2013
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by King Abdullah University for Science and Technology Global Collaborative Partners (GCR) program. YMM was partly funded by an Al-Alfi Biotechnology Fellowship.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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https://repository.kaust.edu.sa/bitstream/10754/597001/1/srep03358.pdf

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title = "Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool",

abstract = "The Red Sea Atlantis II brine pool is an extreme environment that displays multiple harsh conditions such as high temperature, high salinity and high concentrations of multiple, toxic heavy metals. The survival of microbes in such an environment by utilizing resistant enzymes makes them an excellent source of extremophilic enzymes. We constructed a fosmid metagenomic library using DNA isolated from the deepest and most secluded layer of this pool. We report the isolation and biochemical characterization of an unusual esterase: EstATII. EstATII is thermophilic (optimum temperature, 65 C), halotolerant (maintains its activity in up to 4.5{\^a}€...M NaCl) and maintains at least 60% of its activity in the presence of a wide spectrum of heavy metals. The combination of biochemical characteristics of the Red Sea Atlantis II brine pool esterase, i.e., halotolerance, thermophilicity and resistance to heavy metals, makes it a potentially useful biocatalyst.",

author = "Mohamed, {Yasmine M.} and Ghazy, {Mohamed A.} and Ahmed Sayed and Amged Ouf and Hamza El-Dorry and Rania Siam",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by King Abdullah University for Science and Technology Global Collaborative Partners (GCR) program. YMM was partly funded by an Al-Alfi Biotechnology Fellowship. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

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doi = "10.1038/srep03358",

language = "English (US)",

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T1 - Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool

AU - Mohamed, Yasmine M.

AU - Ghazy, Mohamed A.

AU - Sayed, Ahmed

AU - Ouf, Amged

AU - El-Dorry, Hamza

AU - Siam, Rania

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by King Abdullah University for Science and Technology Global Collaborative Partners (GCR) program. YMM was partly funded by an Al-Alfi Biotechnology Fellowship. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2013/11/28

Y1 - 2013/11/28

N2 - The Red Sea Atlantis II brine pool is an extreme environment that displays multiple harsh conditions such as high temperature, high salinity and high concentrations of multiple, toxic heavy metals. The survival of microbes in such an environment by utilizing resistant enzymes makes them an excellent source of extremophilic enzymes. We constructed a fosmid metagenomic library using DNA isolated from the deepest and most secluded layer of this pool. We report the isolation and biochemical characterization of an unusual esterase: EstATII. EstATII is thermophilic (optimum temperature, 65 C), halotolerant (maintains its activity in up to 4.5â€...M NaCl) and maintains at least 60% of its activity in the presence of a wide spectrum of heavy metals. The combination of biochemical characteristics of the Red Sea Atlantis II brine pool esterase, i.e., halotolerance, thermophilicity and resistance to heavy metals, makes it a potentially useful biocatalyst.

AB - The Red Sea Atlantis II brine pool is an extreme environment that displays multiple harsh conditions such as high temperature, high salinity and high concentrations of multiple, toxic heavy metals. The survival of microbes in such an environment by utilizing resistant enzymes makes them an excellent source of extremophilic enzymes. We constructed a fosmid metagenomic library using DNA isolated from the deepest and most secluded layer of this pool. We report the isolation and biochemical characterization of an unusual esterase: EstATII. EstATII is thermophilic (optimum temperature, 65 C), halotolerant (maintains its activity in up to 4.5â€...M NaCl) and maintains at least 60% of its activity in the presence of a wide spectrum of heavy metals. The combination of biochemical characteristics of the Red Sea Atlantis II brine pool esterase, i.e., halotolerance, thermophilicity and resistance to heavy metals, makes it a potentially useful biocatalyst.

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C2 - 24285146

SN - 2045-2322

VL - 3

JO - Scientific Reports

JF - Scientific Reports

IS - 1

ER -

Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool

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