TY - JOUR
T1 - Intraspecific hybrids of Arabidopsis thaliana revealed no gross alterations in endopolyploidy, DNA methylation, histone modifications and transcript levels
AU - Moghaddam, Ali Mohammad Banaei
AU - Fuchs, Jörg
AU - Czauderna, Tobias
AU - Houben, Andreas
AU - Mette, Michael Florian
PY - 2010/1
Y1 - 2010/1
N2 - Arabidopsis accessions Col-0 and C24 and their reciprocal hybrids were employed as a model system to investigate the potential relationship between changes in DNA methylation, chromatin structure, endopolyploidization and gene expression in heterotic genotypes. Nucleolus size, endopolyploidization level and distribution of DNA and histone H3 methylation at the microscopic level does not differ between parents and their hybrids. Methylation sensitive amplified polymorphism revealed a largely constant pattern of DNA methylation (97% of signals analyzed) after intraspecific crosses. The parental expression profile of selected genes was maintained in hybrid offspring. No correlation was found between expression pattern and DNA methylation levels at restriction sites within 5′ regulatory regions. Thus, the results revealed only minor changes of chromatin properties and other nuclear features in response to intraspecific hybridization in Arabidopsis thaliana.
AB - Arabidopsis accessions Col-0 and C24 and their reciprocal hybrids were employed as a model system to investigate the potential relationship between changes in DNA methylation, chromatin structure, endopolyploidization and gene expression in heterotic genotypes. Nucleolus size, endopolyploidization level and distribution of DNA and histone H3 methylation at the microscopic level does not differ between parents and their hybrids. Methylation sensitive amplified polymorphism revealed a largely constant pattern of DNA methylation (97% of signals analyzed) after intraspecific crosses. The parental expression profile of selected genes was maintained in hybrid offspring. No correlation was found between expression pattern and DNA methylation levels at restriction sites within 5′ regulatory regions. Thus, the results revealed only minor changes of chromatin properties and other nuclear features in response to intraspecific hybridization in Arabidopsis thaliana.
UR - http://www.scopus.com/inward/record.url?scp=75849126549&partnerID=8YFLogxK
U2 - 10.1007/s00122-009-1127-x
DO - 10.1007/s00122-009-1127-x
M3 - Article
C2 - 19690829
AN - SCOPUS:75849126549
SN - 0040-5752
VL - 120
SP - 215
EP - 226
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
IS - 2
ER -