Interactions between Rotavirus and Suwannee River Organic Matter: Aggregation, Deposition, and Adhesion Force Measurement

Leonardo Gutierrez, Thanh H. Nguyen

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Interactions between rotavirus and Suwannee River natural organic matter (NOM) were studied by time-resolved dynamic light scattering, quartz crystal microbalance, and atomic force microscopy. In NOM-containing NaCl solutions of up to 600 mM, rotavirus suspension remained stable for over 4 h. Atomic force microscopy (AFM) measurement for interaction force decay length at different ionic strengths showed that nonelectrostatic repulsive forces were mainly responsible for eliminating aggregation in NaCl solutions. Aggregation rates of rotavirus in solutions containing 20 mg C/L increased with divalent cation concentration until reaching a critical coagulation concentration of 30 mM CaCl2 or 70 mM MgCl2. Deposition kinetics of rotavirus on NOM-coated silica surface was studied using quartz crystal microbalance. Experimental attachment efficiencies for rotavirus adsorption to NOM-coated surface in MgCl2 solution were lower than in CaCl2 solution at a given divalent cation concentration. Stronger adhesion force was measured for virus-virus and virus-NOM interactions in CaCl2 solution compared to those in MgCl2 or NaCl solutions at the same ionic strength. This study suggested that divalent cation complexation with carboxylate groups in NOM and on virus surface was an important mechanism in the deposition and aggregation kinetics of rotavirus. © 2012 American Chemical Society.
Original languageEnglish (US)
Pages (from-to)8705-8713
Number of pages9
JournalEnvironmental Science & Technology
Volume46
Issue number16
DOIs
StatePublished - Aug 10 2012
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was partially supported by NSF #0954501, the Academic Excellence Alliance program at King Abdullah University of Science and Technology, and the U.S. Department of Energy DE-FG02-07ER46453 and DE-FG02-07ER46471. We also acknowledge Dr. Scott McLaren, Ms. Ofelia Romero, and Mr. Tony Straub.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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