Inter- and Intraspecific Variations of Bacterial Communities Associated with Marine Sponges from San Juan Island, Washington

O. O. Lee, Y. H. Wong, P.-Y. Qian

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56 Scopus citations

Abstract

This study attempted to assess whether conspecific or congeneric sponges around San Juan Island, Washington, harbor specific bacterial communities. We used a combination of culture-independent DNA fingerprinting techniques (terminal restriction fragment length polymorphism and denaturing gradient gel electrophoresis [DGGE]) and culture-dependent approaches. The results indicated that the bacterial communities in the water column consisted of more diverse bacterial ribotypes than and were drastically different from those associated with the sponges. High levels of similarity in sponge-associated bacterial communities were found only in Myxilla incrustans and Haliclona rufescens, while the bacterial communities in Halichondria panicea varied substantially among sites. Certain terminal restriction fragments or DGGE bands were consistently obtained for different individuals of M. incrustans and H. rufescens collected from different sites, suggesting that there are stable or even specific associations of certain bacteria in these two sponges. However, no specific bacterial associations were found for H. panicea or for any one sponge genus. Sequencing of nine DGGE bands resulted in recovery of seven sequences that best matched the sequences of uncultured Proteobacteria. Three of these sequences fell into the sponge-specific sequence clusters previously suggested. An uncultured alphaproteobacterium and a culturable Bacillus sp. were found exclusively in all M. incrustans sponges, while an uncultured gammaproteobacterium was unique to H. rufescens. In contrast, the cultivation approach indicated that sponges contained a large proportion of Firmicutes, especially Bacillus, and revealed large variations in the culturable bacterial communities associated with congeneric and conspecific sponges. This study revealed sponge species-specific but not genus- or site-specific associations between sponges and bacterial communities and emphasized the importance of using a combination of techniques for studying microbial communities.
Original languageEnglish (US)
Pages (from-to)3513-3521
Number of pages9
JournalApplied and Environmental Microbiology
Volume75
Issue number11
DOIs
StatePublished - Apr 10 2009
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): KAUST005-CML.07/08
Acknowledgements: We thank Rob von Soest, Zoological Museum, University of Amsterdam, for identification of sponges, Y. K. Tam for assistance with TRFLP analysis, V. Thiyagarajan for comments on the manuscript, Shawn Arenallo for editing the manuscript, and Friday Harbor Laboratories for providing research facilities and technical support for field work and preliminary laboratory analysis.
This study was supported by a grant from the China Ocean Mineral Resources Research and Development Association (COMRRDA06/07.SC02) and the KAUST Global Academic Partnership Program (KAUST005-CML.07/08) to P.-Y. Qian.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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