Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect

Dana Almohazey; Vijaya Ravinayagam; Widyan Alamoudi; S. Akhtar; H. Dafalla; Hind Nasser AlSuwaidan; Shoruq. T. Almutairi; Hajer Saleh Alghamdi; Sukaina Ahmed Aldamen; M.A. Almessiere; A. Baykal; Ahmed A. Maarouf; B. Rabindran Jermy

doi:10.3390/cancers15030695

Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect

Dana Almohazey, Vijaya Ravinayagam, Widyan Alamoudi, S. Akhtar, H. Dafalla, Hind Nasser AlSuwaidan, Shoruq. T. Almutairi, Hajer Saleh Alghamdi, Sukaina Ahmed Aldamen, M.A. Almessiere, A. Baykal, Ahmed A. Maarouf, B. Rabindran Jermy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

In nanotherapeutics, gaining insight about the drug interaction with the pore architecture and surface functional groups of nanocarriers is crucial to aid in the development of targeted drug delivery. Manganese ferrite impregnated graphene oxide (MnFe2O4/GO) with a two-dimensional sheet and spherical silica with a three-dimensional interconnected porous structure (MnFe2O4/silica) were evaluated for cisplatin release and cytotoxic effects. Characterization studies revealed the presence of Mn2+ species with a variable spinel cubic phase and superparamagnetic effect. We used first principles calculations to study the physisorption of cisplatin on monodispersed silica and on single- and multi-layered GO. The binding energy of cisplatin on silica and single-layer GO was ~1.5 eV, while it was about double that value for the multilayer GO structure. Moreover, we treated MCF-7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) with our nanocomposites and used the cell viability assay MTT. Both nanocomposites significantly reduced the cell viability. Pt4+ species of cisplatin on the spinel ferrite/silica nanocomposite had a better effect on the cytotoxic capability when compared to GO. The EC50 for MnFe2O4/silica/cisplatin and MnFe2O4/GO/cisplatin on MCF-7 was: 48.43 µg/mL and 85.36 µg/mL, respectively. The EC50 for the same conditions on HFF was: 102.92 µg/mL and 102.21 µg/mL, respectively. In addition, immunofluorescence images using c-caspase 3/7, and TEM analysis indicated that treating cells with these nanocomposites resulted in apoptosis as the major mechanism of cell death.

Original language	English (US)
Pages (from-to)	695
Journal	Cancers
Volume	15
Issue number	3
DOIs	https://doi.org/10.3390/cancers15030695
State	Published - Jan 23 2023
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2023-01-30
Acknowledgements: Vijaya Ravinayagam acknowledges the funding obtained from Deanship of Scientific Research, Imam Abdulrahman Bin Faisal University with grant number 2019-120-DSR. The authors would like to acknowledge Dorothy Joy H. Huelar, Janaica Yu Logan, and Edwardson G. Evangelista for their assistance in the nanocomposite preparation and in vitro studies. A. Maarouf would like to acknowledge the use of the resources of the Supercomputing Laboratory at KAUST, Saudi Arabia. B.R.J would like to acknowledge the advanced state of art facilities provided by Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University (IAU), Saudi Arabia.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/cancers15030695

Cite this

Almohazey, D., Ravinayagam, V., Alamoudi, W., Akhtar, S., Dafalla, H., AlSuwaidan, H. N., Almutairi, S. T., Alghamdi, H. S., Aldamen, S. A., Almessiere, M. A., Baykal, A., Maarouf, A. A., & Jermy, B. R. (2023). Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect. Cancers, 15(3), 695. https://doi.org/10.3390/cancers15030695

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title = "Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect",

abstract = "In nanotherapeutics, gaining insight about the drug interaction with the pore architecture and surface functional groups of nanocarriers is crucial to aid in the development of targeted drug delivery. Manganese ferrite impregnated graphene oxide (MnFe2O4/GO) with a two-dimensional sheet and spherical silica with a three-dimensional interconnected porous structure (MnFe2O4/silica) were evaluated for cisplatin release and cytotoxic effects. Characterization studies revealed the presence of Mn2+ species with a variable spinel cubic phase and superparamagnetic effect. We used first principles calculations to study the physisorption of cisplatin on monodispersed silica and on single- and multi-layered GO. The binding energy of cisplatin on silica and single-layer GO was ~1.5 eV, while it was about double that value for the multilayer GO structure. Moreover, we treated MCF-7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) with our nanocomposites and used the cell viability assay MTT. Both nanocomposites significantly reduced the cell viability. Pt4+ species of cisplatin on the spinel ferrite/silica nanocomposite had a better effect on the cytotoxic capability when compared to GO. The EC50 for MnFe2O4/silica/cisplatin and MnFe2O4/GO/cisplatin on MCF-7 was: 48.43 µg/mL and 85.36 µg/mL, respectively. The EC50 for the same conditions on HFF was: 102.92 µg/mL and 102.21 µg/mL, respectively. In addition, immunofluorescence images using c-caspase 3/7, and TEM analysis indicated that treating cells with these nanocomposites resulted in apoptosis as the major mechanism of cell death.",

author = "Dana Almohazey and Vijaya Ravinayagam and Widyan Alamoudi and S. Akhtar and H. Dafalla and AlSuwaidan, {Hind Nasser} and Almutairi, {Shoruq. T.} and Alghamdi, {Hajer Saleh} and Aldamen, {Sukaina Ahmed} and M.A. Almessiere and A. Baykal and Maarouf, {Ahmed A.} and Jermy, {B. Rabindran}",

note = "KAUST Repository Item: Exported on 2023-01-30 Acknowledgements: Vijaya Ravinayagam acknowledges the funding obtained from Deanship of Scientific Research, Imam Abdulrahman Bin Faisal University with grant number 2019-120-DSR. The authors would like to acknowledge Dorothy Joy H. Huelar, Janaica Yu Logan, and Edwardson G. Evangelista for their assistance in the nanocomposite preparation and in vitro studies. A. Maarouf would like to acknowledge the use of the resources of the Supercomputing Laboratory at KAUST, Saudi Arabia. B.R.J would like to acknowledge the advanced state of art facilities provided by Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University (IAU), Saudi Arabia. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

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doi = "10.3390/cancers15030695",

language = "English (US)",

volume = "15",

pages = "695",

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T1 - Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect

AU - Almohazey, Dana

AU - Ravinayagam, Vijaya

AU - Alamoudi, Widyan

AU - Akhtar, S.

AU - Dafalla, H.

AU - AlSuwaidan, Hind Nasser

AU - Almutairi, Shoruq. T.

AU - Alghamdi, Hajer Saleh

AU - Aldamen, Sukaina Ahmed

AU - Almessiere, M.A.

AU - Baykal, A.

AU - Maarouf, Ahmed A.

AU - Jermy, B. Rabindran

N1 - KAUST Repository Item: Exported on 2023-01-30 Acknowledgements: Vijaya Ravinayagam acknowledges the funding obtained from Deanship of Scientific Research, Imam Abdulrahman Bin Faisal University with grant number 2019-120-DSR. The authors would like to acknowledge Dorothy Joy H. Huelar, Janaica Yu Logan, and Edwardson G. Evangelista for their assistance in the nanocomposite preparation and in vitro studies. A. Maarouf would like to acknowledge the use of the resources of the Supercomputing Laboratory at KAUST, Saudi Arabia. B.R.J would like to acknowledge the advanced state of art facilities provided by Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University (IAU), Saudi Arabia. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2023/1/23

Y1 - 2023/1/23

N2 - In nanotherapeutics, gaining insight about the drug interaction with the pore architecture and surface functional groups of nanocarriers is crucial to aid in the development of targeted drug delivery. Manganese ferrite impregnated graphene oxide (MnFe2O4/GO) with a two-dimensional sheet and spherical silica with a three-dimensional interconnected porous structure (MnFe2O4/silica) were evaluated for cisplatin release and cytotoxic effects. Characterization studies revealed the presence of Mn2+ species with a variable spinel cubic phase and superparamagnetic effect. We used first principles calculations to study the physisorption of cisplatin on monodispersed silica and on single- and multi-layered GO. The binding energy of cisplatin on silica and single-layer GO was ~1.5 eV, while it was about double that value for the multilayer GO structure. Moreover, we treated MCF-7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) with our nanocomposites and used the cell viability assay MTT. Both nanocomposites significantly reduced the cell viability. Pt4+ species of cisplatin on the spinel ferrite/silica nanocomposite had a better effect on the cytotoxic capability when compared to GO. The EC50 for MnFe2O4/silica/cisplatin and MnFe2O4/GO/cisplatin on MCF-7 was: 48.43 µg/mL and 85.36 µg/mL, respectively. The EC50 for the same conditions on HFF was: 102.92 µg/mL and 102.21 µg/mL, respectively. In addition, immunofluorescence images using c-caspase 3/7, and TEM analysis indicated that treating cells with these nanocomposites resulted in apoptosis as the major mechanism of cell death.

AB - In nanotherapeutics, gaining insight about the drug interaction with the pore architecture and surface functional groups of nanocarriers is crucial to aid in the development of targeted drug delivery. Manganese ferrite impregnated graphene oxide (MnFe2O4/GO) with a two-dimensional sheet and spherical silica with a three-dimensional interconnected porous structure (MnFe2O4/silica) were evaluated for cisplatin release and cytotoxic effects. Characterization studies revealed the presence of Mn2+ species with a variable spinel cubic phase and superparamagnetic effect. We used first principles calculations to study the physisorption of cisplatin on monodispersed silica and on single- and multi-layered GO. The binding energy of cisplatin on silica and single-layer GO was ~1.5 eV, while it was about double that value for the multilayer GO structure. Moreover, we treated MCF-7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) with our nanocomposites and used the cell viability assay MTT. Both nanocomposites significantly reduced the cell viability. Pt4+ species of cisplatin on the spinel ferrite/silica nanocomposite had a better effect on the cytotoxic capability when compared to GO. The EC50 for MnFe2O4/silica/cisplatin and MnFe2O4/GO/cisplatin on MCF-7 was: 48.43 µg/mL and 85.36 µg/mL, respectively. The EC50 for the same conditions on HFF was: 102.92 µg/mL and 102.21 µg/mL, respectively. In addition, immunofluorescence images using c-caspase 3/7, and TEM analysis indicated that treating cells with these nanocomposites resulted in apoptosis as the major mechanism of cell death.

UR - http://hdl.handle.net/10754/687358

UR - https://www.mdpi.com/2072-6694/15/3/695

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DO - 10.3390/cancers15030695

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C2 - 36765654

SN - 2072-6694

VL - 15

SP - 695

JO - Cancers

JF - Cancers

IS - 3

ER -

Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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