TY - JOUR
T1 - Insight into the genome of Aspergillus fumigatus
T2 - Analysis of a 922 kb region encompassing the nitrate assimilation gene cluster
AU - Pain, Arnab
AU - Woodward, John
AU - Quail, Michael A.
AU - Anderson, Michael J.
AU - Clark, Richard
AU - Collins, Matthew
AU - Fosker, Nigel
AU - Fraser, Audrey
AU - Harris, David
AU - Larke, Natasha
AU - Murphy, Lee
AU - Humphray, Sean
AU - O'Neil, Susan
AU - Pertea, Mihaela
AU - Price, Claire
AU - Rabbinowitsch, Ester
AU - Rajandream, Marie Adele
AU - Salzberg, Steven
AU - Saunders, David
AU - Seeger, Kathy
AU - Sharp, Sarah
AU - Warren, Tim
AU - Denning, David W.
AU - Barrell, Bart
AU - Hall, Neil
PY - 2004/4/1
Y1 - 2004/4/1
N2 - Aspergillus fumigatus is the most ubiquitous opportunistic filamentous fungal pathogen of human. As an initial step toward sequencing the entire genome of A. fumigatus, which is estimated to be ∼30Mb in size, we have sequenced a 922kb region, contained within 16 overlapping bacterial artificial chromosome (BAC) clones. Fifty-four percent of the DNA is predicted to be coding with 341 putative protein coding genes. Functional classification of the proteins showed the presence of a higher proportion of enzymes and membrane transporters when compared to those of Saccharomyces cerevisiae. In addition to the nitrate assimilation gene cluster, the quinate utilisation gene cluster is also present on this 922kb genomic sequence. We observed large scale synteny between A. fumigatus and Aspergillus nidulans by comparing this sequence to the A. nidulans genetic map of linkage group VIII.
AB - Aspergillus fumigatus is the most ubiquitous opportunistic filamentous fungal pathogen of human. As an initial step toward sequencing the entire genome of A. fumigatus, which is estimated to be ∼30Mb in size, we have sequenced a 922kb region, contained within 16 overlapping bacterial artificial chromosome (BAC) clones. Fifty-four percent of the DNA is predicted to be coding with 341 putative protein coding genes. Functional classification of the proteins showed the presence of a higher proportion of enzymes and membrane transporters when compared to those of Saccharomyces cerevisiae. In addition to the nitrate assimilation gene cluster, the quinate utilisation gene cluster is also present on this 922kb genomic sequence. We observed large scale synteny between A. fumigatus and Aspergillus nidulans by comparing this sequence to the A. nidulans genetic map of linkage group VIII.
KW - Annotation
KW - Aspergillus fumigatus
KW - BAC-end sequencing
KW - Comparative genomics
KW - Gene-cluster
KW - Genome
KW - Synteny
UR - http://www.scopus.com/inward/record.url?scp=10744221599&partnerID=8YFLogxK
U2 - 10.1016/j.fgb.2003.12.003
DO - 10.1016/j.fgb.2003.12.003
M3 - Article
C2 - 14998527
AN - SCOPUS:10744221599
VL - 41
SP - 443
EP - 453
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
IS - 4
ER -