Aspergillus fumigatus is the most ubiquitous opportunistic filamentous fungal pathogen of human. As an initial step toward sequencing the entire genome of A. fumigatus, which is estimated to be ∼30Mb in size, we have sequenced a 922kb region, contained within 16 overlapping bacterial artificial chromosome (BAC) clones. Fifty-four percent of the DNA is predicted to be coding with 341 putative protein coding genes. Functional classification of the proteins showed the presence of a higher proportion of enzymes and membrane transporters when compared to those of Saccharomyces cerevisiae. In addition to the nitrate assimilation gene cluster, the quinate utilisation gene cluster is also present on this 922kb genomic sequence. We observed large scale synteny between A. fumigatus and Aspergillus nidulans by comparing this sequence to the A. nidulans genetic map of linkage group VIII.
Bibliographical noteFunding Information:
We are indebted to Rod Warren for having the forethought to save the clinical isolate that was selected for sequencing and for providing extensive clinical details on this strain. Also thanks to Caroline Moore for susceptibility testing, to Stephan Bretagne for microsattelite typing, to David Geiser for his phylogenetic analysis, to Mike Bromley for providing the niaD gene sequence and to Jonathon Ross for performing the orthology analysis between A. fumigatus and A. nidulans. We are also indebted to the Wellcome Trust and the Fungal Research Trust for funding the work. We are grateful to Hubert Renauld for advice and corrections to the manuscript.
- Aspergillus fumigatus
- BAC-end sequencing
- Comparative genomics
ASJC Scopus subject areas