Influences of Diurnal Sampling Bias on Fixed-Point Monitoring of Plankton Biodiversity Determined Using a Massively Parallel Sequencing-Based Technique

Satoshi Nagai; Noriko Nishi; Shingo Urushizaki; Goh Onitsuka; Motoshige Yasuike; Yoji Nakamura; Atushi Fujiwara; Seisuke Tajimi; Takanori Kobayashi; Takashi Gojobori; Mitsuru Ototake

doi:10.1007/978-981-13-8134-8_14

Influences of Diurnal Sampling Bias on Fixed-Point Monitoring of Plankton Biodiversity Determined Using a Massively Parallel Sequencing-Based Technique

Satoshi Nagai, Noriko Nishi, Shingo Urushizaki, Goh Onitsuka, Motoshige Yasuike, Yoji Nakamura, Atushi Fujiwara, Seisuke Tajimi, Takanori Kobayashi, Takashi Gojobori, Mitsuru Ototake

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

In this study, we investigated the influence of diurnal sampling bias on the community structure of plankton by comparing the biodiversity among seawater samples (n = 9) obtained every 3 h for 24 h by using massively parallel sequencing (MPS)-based plankton monitoring at a fixed point conducted at Himedo seaport in Yatsushiro Sea, Japan. During seawater sampling, the semidiurnal tidal current having an amplitude of 0.3 m s−1 was dominant, and the westward residual current driven by the northeasterly wind was continuously observed during the 24-h monitoring. Therefore, the relative abundance of plankton species apparently fluctuated among the samples, but no significant difference was noted according to G-test (p > 0.05). Significant differences were observed between the samples obtained from a different locality and at different dates, suggesting that the influence of diurnal sampling bias on plankton diversity is acceptable and data taken at a certain time in a day can be used as the representative one.

Original language	English (US)
Title of host publication	Marine Metagenomics
Publisher	Springer Singapore
Pages	231-248
Number of pages	18
ISBN (Print)	9789811381331
DOIs	https://doi.org/10.1007/978-981-13-8134-8_14
State	Published - Jul 24 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This study was supported by a Grant-in-Aid (Marine Metagenomics for Monitoring the Coastal Microbiota) from the Ministry of Agriculture, Forestry and Fisheries of Japan.

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10.1007/978-981-13-8134-8_14

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Nagai, S., Nishi, N., Urushizaki, S., Onitsuka, G., Yasuike, M., Nakamura, Y., Fujiwara, A., Tajimi, S., Kobayashi, T., Gojobori, T., & Ototake, M. (2019). Influences of Diurnal Sampling Bias on Fixed-Point Monitoring of Plankton Biodiversity Determined Using a Massively Parallel Sequencing-Based Technique. In Marine Metagenomics (pp. 231-248). Springer Singapore. https://doi.org/10.1007/978-981-13-8134-8_14

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title = "Influences of Diurnal Sampling Bias on Fixed-Point Monitoring of Plankton Biodiversity Determined Using a Massively Parallel Sequencing-Based Technique",

abstract = "In this study, we investigated the influence of diurnal sampling bias on the community structure of plankton by comparing the biodiversity among seawater samples (n = 9) obtained every 3 h for 24 h by using massively parallel sequencing (MPS)-based plankton monitoring at a fixed point conducted at Himedo seaport in Yatsushiro Sea, Japan. During seawater sampling, the semidiurnal tidal current having an amplitude of 0.3 m s−1 was dominant, and the westward residual current driven by the northeasterly wind was continuously observed during the 24-h monitoring. Therefore, the relative abundance of plankton species apparently fluctuated among the samples, but no significant difference was noted according to G-test (p > 0.05). Significant differences were observed between the samples obtained from a different locality and at different dates, suggesting that the influence of diurnal sampling bias on plankton diversity is acceptable and data taken at a certain time in a day can be used as the representative one.",

author = "Satoshi Nagai and Noriko Nishi and Shingo Urushizaki and Goh Onitsuka and Motoshige Yasuike and Yoji Nakamura and Atushi Fujiwara and Seisuke Tajimi and Takanori Kobayashi and Takashi Gojobori and Mitsuru Ototake",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This study was supported by a Grant-in-Aid (Marine Metagenomics for Monitoring the Coastal Microbiota) from the Ministry of Agriculture, Forestry and Fisheries of Japan.",

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doi = "10.1007/978-981-13-8134-8_14",

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Nagai, S, Nishi, N, Urushizaki, S, Onitsuka, G, Yasuike, M, Nakamura, Y, Fujiwara, A, Tajimi, S, Kobayashi, T, Gojobori, T & Ototake, M 2019, Influences of Diurnal Sampling Bias on Fixed-Point Monitoring of Plankton Biodiversity Determined Using a Massively Parallel Sequencing-Based Technique. in Marine Metagenomics. Springer Singapore, pp. 231-248. https://doi.org/10.1007/978-981-13-8134-8_14

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T1 - Influences of Diurnal Sampling Bias on Fixed-Point Monitoring of Plankton Biodiversity Determined Using a Massively Parallel Sequencing-Based Technique

AU - Nagai, Satoshi

AU - Nishi, Noriko

AU - Urushizaki, Shingo

AU - Onitsuka, Goh

AU - Yasuike, Motoshige

AU - Nakamura, Yoji

AU - Fujiwara, Atushi

AU - Tajimi, Seisuke

AU - Kobayashi, Takanori

AU - Gojobori, Takashi

AU - Ototake, Mitsuru

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This study was supported by a Grant-in-Aid (Marine Metagenomics for Monitoring the Coastal Microbiota) from the Ministry of Agriculture, Forestry and Fisheries of Japan.

PY - 2019/7/24

Y1 - 2019/7/24

N2 - In this study, we investigated the influence of diurnal sampling bias on the community structure of plankton by comparing the biodiversity among seawater samples (n = 9) obtained every 3 h for 24 h by using massively parallel sequencing (MPS)-based plankton monitoring at a fixed point conducted at Himedo seaport in Yatsushiro Sea, Japan. During seawater sampling, the semidiurnal tidal current having an amplitude of 0.3 m s−1 was dominant, and the westward residual current driven by the northeasterly wind was continuously observed during the 24-h monitoring. Therefore, the relative abundance of plankton species apparently fluctuated among the samples, but no significant difference was noted according to G-test (p > 0.05). Significant differences were observed between the samples obtained from a different locality and at different dates, suggesting that the influence of diurnal sampling bias on plankton diversity is acceptable and data taken at a certain time in a day can be used as the representative one.

AB - In this study, we investigated the influence of diurnal sampling bias on the community structure of plankton by comparing the biodiversity among seawater samples (n = 9) obtained every 3 h for 24 h by using massively parallel sequencing (MPS)-based plankton monitoring at a fixed point conducted at Himedo seaport in Yatsushiro Sea, Japan. During seawater sampling, the semidiurnal tidal current having an amplitude of 0.3 m s−1 was dominant, and the westward residual current driven by the northeasterly wind was continuously observed during the 24-h monitoring. Therefore, the relative abundance of plankton species apparently fluctuated among the samples, but no significant difference was noted according to G-test (p > 0.05). Significant differences were observed between the samples obtained from a different locality and at different dates, suggesting that the influence of diurnal sampling bias on plankton diversity is acceptable and data taken at a certain time in a day can be used as the representative one.

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EP - 248

BT - Marine Metagenomics

PB - Springer Singapore

ER -

Influences of Diurnal Sampling Bias on Fixed-Point Monitoring of Plankton Biodiversity Determined Using a Massively Parallel Sequencing-Based Technique

Abstract

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