Influence of the Fabrication Accuracy of Hot-Embossed PCL Scaffolds on Cell Growths.

Tania Limongi, Elisabetta Dattola, Cirino Botta, Maria Laura Coluccio, Patrizio Candeloro, Maria Cucè, Bernadette Scopacasa, Maria Eugenia Gallo Cantafio, Costantino Davide Critello, Salvatore Andrea Pullano, Antonino S. Fiorillo, Pierosandro Tagliaferri, Pierfrancesco Tassone, Ernesto Lamanna, Enzo M. Di Fabrizio, Gerardo Perozziello

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Polycaprolactone (PCL) is a biocompatible and biodegradable polymer widely used for the realization of 3D scaffold for tissue engineering applications. The hot embossing technique (HE) allows the obtainment of PCL scaffolds with a regular array of micro pillars on their surface. The main drawback affecting this kind of micro fabrication process is that such structural superficial details can be damaged when detaching the replica from the mold. Therefore, the present study has focused on the optimization of the HE processes through the development of an analytical model for the prediction of the demolding force as a function of temperature. This model allowed calculating the minimum demolding force to obtain regular micropillars without defects. We demonstrated that the results obtained by the analytical model agree with the experimental data. To address the importance of controlling accurately the fabricated microstructures, we seeded on the PCL scaffolds human stromal cell line (HS-5) and monocytic leukemia cell line (THP-1) to evaluate how the presence of regular or deformed pillars affect cells viability. In vitro viability results, scanning electron and fluorescence microscope imaging analysis show that the HS-5 preferentially grows on regular microstructured surfaces, while the THP-1 on irregular microstructured ones.
Original languageEnglish (US)
JournalFrontiers in Bioengineering and Biotechnology
StatePublished - Mar 1 2020

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the project for young researchers financed from the Ministry of Health “High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices” (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University “Magna Graecia” of Catanzaro.


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