Inactivation and Gene Expression of a Virulent WastewaterEscherichia coliStrain and the Nonvirulent CommensalEscherichia coliDSM1103 Strain upon Solar Irradiation

Nada I. Aljassim, David Mantilla Calderon, Tiannyu Wang, Pei-Ying Hong

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

This study examined the decay kinetics and molecular responses of two Escherichia coli strains upon solar irradiation. The first is E. coli PI-7, a virulent and antibiotic-resistant strain that was isolated from wastewater and carries the emerging NDM-1 antibiotic resistance gene. The other strain, E. coli DSM1103, displayed lower virulence and antibiotic resistance than E. coli PI-7. In a buffer solution, E. coli PI-7 displayed a longer lag phase prior to decay and a longer half-life compared with E. coli DSM1103 (6.64 ± 0.63 h and 2.85 ± 0.46 min vs 1.33 ± 0.52 h and 2.04 ± 0.36 min). In wastewater, both E. coli strains decayed slower than they did in buffer. Although solar irradiation remained effective in reducing the numbers of both strains by more than 5-log10 in
Original languageEnglish (US)
Pages (from-to)3649-3659
Number of pages11
JournalEnvironmental Science & Technology
Volume51
Issue number7
DOIs
StatePublished - Mar 17 2017

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): BAS/1/1033-01-01
Acknowledgements: This study is supported by KAUST baseline funding BAS/1/ 1033-01-01 awarded to P.-Y. Hong. The authors thank Dr. Shengkun Dong and Professor Helen Nguyen for providing training to N.A.-J. on the use of the solar simulator in the earlier phase of this project; Dr. Inhyuk Kwon and Professor Roderick Mackie for providing training to N.A.-J. on transcriptomics analysis; and Dr. Muhammad Raihan Jumat for providing assistance with reverse transcription. The authors express gratitude to Mr. George Princeton Dunsford for granting access to the wastewater samples and to Dr. Moataz El Ghany for ideas pertaining to E. coli transcriptomics.

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