Abstract
A new β-microprobe (βP) has been used to locally measure the time-concentration curve of a radiolabeled substance. The βP, analogous to positron emission tomography methodology, is useful for in vivo animal studies because it can acquire time-concentration curves with high temporal and spatial resolution. Using [ 18F]fluoro-2-deoxy-D-glucose and βP, we evaluated the reliability of the biologic parameters and we compared this method with the [ 14C]2-deoxy-D-glucose autoradiography. βP time-concentration curves in three regions of the brain were obtained from 24 rats. Four kinetic parameters (K 1-k 4) were estimated from 60-minute experimental periods using a three-compartment model. Best fits were obtained when the vascular fraction (F v) was estimated simultaneously with the four kinetic parameters (K 1-k 4). The mean estimated F v values were about 5.5% for the frontal cortex regions and 8.0% for the cerebellum. Correlation coefficients higher than 0.830 were observed between regional cerebral metabolic rates for glucose (rCMRglc) values obtained by βP and autoradiography. In addition, the βP-derived input function was similar to that obtained by manual sampling. Our findings show that reliable rCMRglc values can be obtained using βP.
Original language | English (US) |
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Pages (from-to) | 1015-1024 |
Number of pages | 10 |
Journal | Journal of Cerebral Blood Flow and Metabolism |
Volume | 24 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2004 |
Externally published | Yes |
Keywords
- Autoradiography
- Mathematical modeling
- rCMRglc
- β-microprobe
ASJC Scopus subject areas
- Neurology
- Clinical Neurology
- Cardiology and Cardiovascular Medicine