In-Vivo fluorescent nanosensor implants based on hydrogel-encapsulation: investigating the inflammation and the foreign-body response

Michael A. Lee, Xiaojia Jin, Sureshkumar Muthupalani, Naveed A. Bakh, Xun Gong, Michael S. Strano

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Nanotechnology-enabled sensors or nanosensors are emerging as promising new tools for various in-vivo life science applications such as biosensing, components of delivery systems, and probes for spatial bioimaging. However, as with a wide range of synthetic biomaterials, tissue responses have been observed depending on cell types and various nanocomponent properties. The tissue response is critical for determining the acute and long term health of the organism and the functional lifetime of the material in-vivo. While nanomaterial properties can contribute significantly to the tissue response, it may be possible to circumvent adverse reactions by formulation of the encapsulation vehicle. In this study, five formulations of poly (ethylene glycol) diacrylate (PEGDA) hydrogel-encapsulated fluorescent nanosensors were implanted into SKH-1E mice, and the inflammatory responses were tracked in order to determine the favorable design rules for hydrogel encapsulation and minimization of such responses. Hydrogels with higher crosslinking density were found to allow faster resolution of acute inflammation. Five different immunocompromised mice lines were utilized for comparison across different inflammatory cell populations and responses. Degradation products of the gels were also characterized. Finally, the importance of the tissue response in determining functional lifetime was demonstrated by measuring the time-dependent nanosensor deactivation following implantation into animal models.
Original languageEnglish (US)
JournalJournal of Nanobiotechnology
Volume21
Issue number1
DOIs
StatePublished - Apr 24 2023
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2023-05-03
Acknowledged KAUST grant number(s): OSR-2015 Sensors 2707
Acknowledgements: This research was supported by the King Abdullah University of Science & Technology (OSR-2015 Sensors 2707).
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

ASJC Scopus subject areas

  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Pharmaceutical Science
  • Molecular Medicine
  • Medicine (miscellaneous)

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