In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties

Philippe Thueler; Igor Charvet; Frederic Bevilacqua; M. St Ghislain; G. Ory; Pierre Marquet; Paolo Meda; Ben Vermeulen; Christian Depeursinge

doi:10.1117/1.1578494

In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties

Philippe Thueler^*, Igor Charvet, Frederic Bevilacqua, M. St Ghislain, G. Ory, Pierre Marquet, Paolo Meda, Ben Vermeulen, Christian Depeursinge

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

136 Scopus citations

Abstract

A fast spectroscopic system for superficial and local determination of the absorption and scattering properties of tissue (480 to 950 nm) is described. The probe can be used in the working channel of an endoscope. The scattering properties include the reduced scattering coefficient and a parameter of the phase function called γ, which depends on its first two moments. The inverse problem algorithm is based on the fit of absolute reflectance measurements to cubic B-spline functions derived from the interpolation of a set of Monte Carlo simulations. The algorithm's robustness was tested with simulations altered with various amounts of noise. The method was also assessed on tissue phantoms of known optical properties. Finally, clinical measurements performed endoscopically in vivo in the stomach of human subjects are presented. The absorption and scattering properties were found to be significantly different in the antrum and in the fundus and are correlated with histopathologic observations. The method and the instrument show promise for noninvasive tissue diagnostics of various epithelia.

Original language	English (US)
Pages (from-to)	495-503
Number of pages	9
Journal	Journal of biomedical optics
Volume	8
Issue number	3
DOIs	https://doi.org/10.1117/1.1578494
State	Published - Jul 2003
Externally published	Yes

Bibliographical note

Funding Information:
We are grateful to M. A. Brundler for the histological analysis. We thank P. Marquet for his help in the project and A. Munoz, P. Thevenaz, and M. Unser for the spline interpolation. We thank the Common Program in Biomedical Engineering of the HUG-UNIL-EPFL-UNIGE-HCV, project MI-CRODIAG for their financial support. F. Bevilacqua gratefully acknowledges support from the Swiss National Science Foundation and from the Laser Microbeam and Medical Program of the U.S. National Institutes of Health (grant RR01192).

Keywords

Endoscopy
Gastroenterology
Optical biopsy
Photon migration
Spectroscopy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomedical Engineering
Biomaterials

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10.1117/1.1578494

Cite this

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title = "In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties",

abstract = "A fast spectroscopic system for superficial and local determination of the absorption and scattering properties of tissue (480 to 950 nm) is described. The probe can be used in the working channel of an endoscope. The scattering properties include the reduced scattering coefficient and a parameter of the phase function called γ, which depends on its first two moments. The inverse problem algorithm is based on the fit of absolute reflectance measurements to cubic B-spline functions derived from the interpolation of a set of Monte Carlo simulations. The algorithm's robustness was tested with simulations altered with various amounts of noise. The method was also assessed on tissue phantoms of known optical properties. Finally, clinical measurements performed endoscopically in vivo in the stomach of human subjects are presented. The absorption and scattering properties were found to be significantly different in the antrum and in the fundus and are correlated with histopathologic observations. The method and the instrument show promise for noninvasive tissue diagnostics of various epithelia.",

keywords = "Endoscopy, Gastroenterology, Optical biopsy, Photon migration, Spectroscopy",

author = "Philippe Thueler and Igor Charvet and Frederic Bevilacqua and Ghislain, {M. St} and G. Ory and Pierre Marquet and Paolo Meda and Ben Vermeulen and Christian Depeursinge",

note = "Funding Information: We are grateful to M. A. Brundler for the histological analysis. We thank P. Marquet for his help in the project and A. Munoz, P. Thevenaz, and M. Unser for the spline interpolation. We thank the Common Program in Biomedical Engineering of the HUG-UNIL-EPFL-UNIGE-HCV, project MI-CRODIAG for their financial support. F. Bevilacqua gratefully acknowledges support from the Swiss National Science Foundation and from the Laser Microbeam and Medical Program of the U.S. National Institutes of Health (grant RR01192).",

year = "2003",

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doi = "10.1117/1.1578494",

language = "English (US)",

volume = "8",

pages = "495--503",

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AU - Thueler, Philippe

AU - Charvet, Igor

AU - Bevilacqua, Frederic

AU - Ghislain, M. St

AU - Ory, G.

AU - Marquet, Pierre

AU - Meda, Paolo

AU - Vermeulen, Ben

AU - Depeursinge, Christian

N1 - Funding Information: We are grateful to M. A. Brundler for the histological analysis. We thank P. Marquet for his help in the project and A. Munoz, P. Thevenaz, and M. Unser for the spline interpolation. We thank the Common Program in Biomedical Engineering of the HUG-UNIL-EPFL-UNIGE-HCV, project MI-CRODIAG for their financial support. F. Bevilacqua gratefully acknowledges support from the Swiss National Science Foundation and from the Laser Microbeam and Medical Program of the U.S. National Institutes of Health (grant RR01192).

PY - 2003/7

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N2 - A fast spectroscopic system for superficial and local determination of the absorption and scattering properties of tissue (480 to 950 nm) is described. The probe can be used in the working channel of an endoscope. The scattering properties include the reduced scattering coefficient and a parameter of the phase function called γ, which depends on its first two moments. The inverse problem algorithm is based on the fit of absolute reflectance measurements to cubic B-spline functions derived from the interpolation of a set of Monte Carlo simulations. The algorithm's robustness was tested with simulations altered with various amounts of noise. The method was also assessed on tissue phantoms of known optical properties. Finally, clinical measurements performed endoscopically in vivo in the stomach of human subjects are presented. The absorption and scattering properties were found to be significantly different in the antrum and in the fundus and are correlated with histopathologic observations. The method and the instrument show promise for noninvasive tissue diagnostics of various epithelia.

AB - A fast spectroscopic system for superficial and local determination of the absorption and scattering properties of tissue (480 to 950 nm) is described. The probe can be used in the working channel of an endoscope. The scattering properties include the reduced scattering coefficient and a parameter of the phase function called γ, which depends on its first two moments. The inverse problem algorithm is based on the fit of absolute reflectance measurements to cubic B-spline functions derived from the interpolation of a set of Monte Carlo simulations. The algorithm's robustness was tested with simulations altered with various amounts of noise. The method was also assessed on tissue phantoms of known optical properties. Finally, clinical measurements performed endoscopically in vivo in the stomach of human subjects are presented. The absorption and scattering properties were found to be significantly different in the antrum and in the fundus and are correlated with histopathologic observations. The method and the instrument show promise for noninvasive tissue diagnostics of various epithelia.

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KW - Photon migration

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In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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