In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties

Philippe Thueler*, Igor Charvet, Frederic Bevilacqua, M. St Ghislain, G. Ory, Pierre Marquet, Paolo Meda, Ben Vermeulen, Christian Depeursinge

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

136 Scopus citations


A fast spectroscopic system for superficial and local determination of the absorption and scattering properties of tissue (480 to 950 nm) is described. The probe can be used in the working channel of an endoscope. The scattering properties include the reduced scattering coefficient and a parameter of the phase function called γ, which depends on its first two moments. The inverse problem algorithm is based on the fit of absolute reflectance measurements to cubic B-spline functions derived from the interpolation of a set of Monte Carlo simulations. The algorithm's robustness was tested with simulations altered with various amounts of noise. The method was also assessed on tissue phantoms of known optical properties. Finally, clinical measurements performed endoscopically in vivo in the stomach of human subjects are presented. The absorption and scattering properties were found to be significantly different in the antrum and in the fundus and are correlated with histopathologic observations. The method and the instrument show promise for noninvasive tissue diagnostics of various epithelia.

Original languageEnglish (US)
Pages (from-to)495-503
Number of pages9
JournalJournal of biomedical optics
Issue number3
StatePublished - Jul 2003
Externally publishedYes

Bibliographical note

Funding Information:
We are grateful to M. A. Brundler for the histological analysis. We thank P. Marquet for his help in the project and A. Munoz, P. Thevenaz, and M. Unser for the spline interpolation. We thank the Common Program in Biomedical Engineering of the HUG-UNIL-EPFL-UNIGE-HCV, project MI-CRODIAG for their financial support. F. Bevilacqua gratefully acknowledges support from the Swiss National Science Foundation and from the Laser Microbeam and Medical Program of the U.S. National Institutes of Health (grant RR01192).


  • Endoscopy
  • Gastroenterology
  • Optical biopsy
  • Photon migration
  • Spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Biomaterials


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