In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture

Shannon Klein, Samhan M. Alsolami, Silvia Arossa, Gerardo Ramos Mandujano, Anieka Parry, Alexandra Steckbauer, Carlos M. Duarte, Mo Li

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

AbstractMammalian cell cultures are a keystone resource in biomedical research, but the results of published experiments often suffer from reproducibility challenges. This has led to a focus on the influence of cell culture conditions on cellular responses and reproducibility of experimental findings. Here, we perform frequent in situ monitoring of dissolved O2 and CO2 with optical sensor spots and contemporaneous evaluation of cell proliferation and medium pH in standard batch cultures of three widely used human somatic and pluripotent stem cell lines. We collate data from the literature to demonstrate that standard cell cultures consistently exhibit environmental instability, indicating that this may be a pervasive issue affecting experimental findings. Our results show that in vitro cell cultures consistently undergo large departures of environmental parameters during standard batch culture. These findings should catalyze further efforts to increase the relevance of experimental results to the in vivo physiology and enhance reproducibility.
Original languageEnglish (US)
JournalCommunications Biology
Volume5
Issue number1
DOIs
StatePublished - Feb 8 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-02-11
Acknowledged KAUST grant number(s): BAS/1/1080-01, URF/1/3412-01
Acknowledgements: KAUST funded this research through funding from the Office of Sponsored Research award numbers BAS/1/1080-01 and URF/1/3412-01-01 to ML and baseline funding to CMD. Figure 1 was created by Heno Hwang, scientific illustrator at KAUST.

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