In-silico detection of aneuploidy and chromosomal deletions in wheat using genotyping-by-sequencing

Narinder Singh, John Raupp, Dal Hoe Koo, Bernd Friebe, Bikram Gill, Jesse Poland

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Short read sequencing technologies, such as genotyping-by-sequencing (GBS), have been utilized in genetic mapping, marker development, and population genomic studies. High-throughput and multiplexing capability coupled with low cost make GBS an appropriate tool for molecular research. Here, we present the application of GBS to characterize wheat aneuploid stocks and detect chromosomal aberrations including aneuploidy and chromosomal deletions. These aneuploids are an important resource that have been used in wheat genetics and genomics studies to localize genes, determine physical positions, and develop chromosome bin maps. Results: Using GBS, we mapped sequence reads and quantified read coverage across chromosome bins. Using this approach, we confirmed known deletions and aneuploid stocks. In addition, we were also able to fully characterize these stocks and to identify several novel deletions and aneuploids. With this knowledge and a quick detection tool at our disposal, we can easily isolate these deletions and aneuploids into distinct lines. Conclusion: We envision this tool to replace the intensive cytogenetics techniques, such as C-banding, and fluorescent- and genomic-in situ hybridization to accurately detect chromosome dosage and segmental deletions in wheat genetic stocks as well as other crop species.
Original languageEnglish (US)
JournalPlant Methods
Volume16
Issue number1
DOIs
StatePublished - Apr 6 2020
Externally publishedYes

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Biotechnology

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