In-line balanced detection stimulated Raman scattering microscopy

Francesco Crisafi, Vikas Kumar, Tullio Scopigno, Marco Marangoni, Giulio Cerullo, Dario Polli

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.
Original languageEnglish (US)
JournalScientific Reports
Issue number1
StatePublished - Sep 6 2017
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work has been supported by European Research Council Consolidator Grant VIBRA (ERC-2014-CoG 648615), Horizon2020 GRAPHENE Flagship (696656) and KAUST (OSR-2016-CRG5-3017-01). We thank Andrea Ragni for his help in detector noise measurements and calculations. Abigail D.G. Nunn, L. Belloni and N. Pediconi are kindly acknowledged for providing HepaRG and HuH7 cell lines.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.


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