In-line balanced detection stimulated Raman scattering microscopy

Francesco Crisafi; Vikas Kumar; Tullio Scopigno; Marco Marangoni; Giulio Cerullo; Dario Polli

doi:10.1038/s41598-017-09839-1

In-line balanced detection stimulated Raman scattering microscopy

Francesco Crisafi, Vikas Kumar, Tullio Scopigno, Marco Marangoni, Giulio Cerullo, Dario Polli

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.

Original language	English (US)
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-09839-1
State	Published - Sep 6 2017
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work has been supported by European Research Council Consolidator Grant VIBRA (ERC-2014-CoG 648615), Horizon2020 GRAPHENE Flagship (696656) and KAUST (OSR-2016-CRG5-3017-01). We thank Andrea Ragni for his help in detector noise measurements and calculations. Abigail D.G. Nunn, L. Belloni and N. Pediconi are kindly acknowledged for providing HepaRG and HuH7 cell lines.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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10.1038/s41598-017-09839-1

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title = "In-line balanced detection stimulated Raman scattering microscopy",

abstract = "We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.",

author = "Francesco Crisafi and Vikas Kumar and Tullio Scopigno and Marco Marangoni and Giulio Cerullo and Dario Polli",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work has been supported by European Research Council Consolidator Grant VIBRA (ERC-2014-CoG 648615), Horizon2020 GRAPHENE Flagship (696656) and KAUST (OSR-2016-CRG5-3017-01). We thank Andrea Ragni for his help in detector noise measurements and calculations. Abigail D.G. Nunn, L. Belloni and N. Pediconi are kindly acknowledged for providing HepaRG and HuH7 cell lines. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

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doi = "10.1038/s41598-017-09839-1",

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T1 - In-line balanced detection stimulated Raman scattering microscopy

AU - Crisafi, Francesco

AU - Kumar, Vikas

AU - Scopigno, Tullio

AU - Marangoni, Marco

AU - Cerullo, Giulio

AU - Polli, Dario

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work has been supported by European Research Council Consolidator Grant VIBRA (ERC-2014-CoG 648615), Horizon2020 GRAPHENE Flagship (696656) and KAUST (OSR-2016-CRG5-3017-01). We thank Andrea Ragni for his help in detector noise measurements and calculations. Abigail D.G. Nunn, L. Belloni and N. Pediconi are kindly acknowledged for providing HepaRG and HuH7 cell lines. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2017/9/6

Y1 - 2017/9/6

N2 - We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.

AB - We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.

UR - http://hdl.handle.net/10754/625800

UR - http://www.nature.com/articles/s41598-017-09839-1

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DO - 10.1038/s41598-017-09839-1

M3 - Article

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

IS - 1

ER -

In-line balanced detection stimulated Raman scattering microscopy

Abstract

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