TY - JOUR
T1 - Imaging intracellular fluorescent proteins at nanometer resolution
AU - Betzig, Eric
AU - Patterson, George H.
AU - Sougrat, Rachid
AU - Lindwasser, O. Wolf
AU - Olenych, Scott
AU - Bonifacino, Juan S.
AU - Davidson, Michael W.
AU - Lippincott-Schwartz, Jennifer
AU - Hess, Harald F.
PY - 2006/9/15
Y1 - 2006/9/15
N2 - We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ∼2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method-termed photoactivated localization microscopy-to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.
AB - We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ∼2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method-termed photoactivated localization microscopy-to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.
UR - http://www.scopus.com/inward/record.url?scp=33747179417&partnerID=8YFLogxK
U2 - 10.1126/science.1127344
DO - 10.1126/science.1127344
M3 - Article
C2 - 16902090
AN - SCOPUS:33747179417
SN - 0036-8075
VL - 313
SP - 1642
EP - 1645
JO - SCIENCE
JF - SCIENCE
IS - 5793
ER -