Identification of self-consistent modulons from bacterial microarray expression data with the help of structured regulon gene sets

Elizaveta A. Permina, Yulia Medvedeva, Pia M. Baeck, Shubhada R. Hegde, Shekhar C. Mande, Vsevolod J. Makeev

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1 Scopus citations


Identification of bacterial modulons from series of gene expression measurements on microarrays is a principal problem, especially relevant for inadequately studied but practically important species. Usage of a priori information on regulatory interactions helps to evaluate parameters for regulatory subnetwork inference. We suggest a procedure for modulon construction where a seed regulon is iteratively updated with genes having expression patterns similar to those for regulon member genes. A set of genes essential for a regulon is used to control modulon updating. Essential genes for a regulon were selected as a subset of regulon genes highly related by different measures to each other. Using Escherichia coli as a model, we studied how modulon identification depends on the data, including the microarray experiments set, the adopted relevance measure and the regulon itself. We have found that results of modulon identification are highly dependent on all parameters studied and thus the resulting modulon varies substantially depending on the identification procedure. Yet, modulons that were identified correctly displayed higher stability during iterations, which allows developing a procedure for reliable modulon identification in the case of less studied species where the known regulatory interactions are sparse. Copyright © 2013 Taylor & Francis.
Original languageEnglish (US)
Pages (from-to)115-124
Number of pages10
JournalJournal of Biomolecular Structure and Dynamics
Issue number1
StatePublished - Jan 2013

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We are grateful to Dmitri Ravcheev for the fruitful consultation and for information provided about PurR regulon and Alexander Favorov for kindly providing a module for calculation of exact Fisher test. This study was partially supported with Russian Fund of Basic Research grant 10-04-92,663-IND-a and Department of Science and Technology, India grant INT/RFBR/P-31.

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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