Identification of methanogenesis and syntrophy as important microbial metabolic processes for optimal thermophilic anaerobic digestion of energy cane thin stillage

Margreet J. Oosterkamp; Stefan Bauer; Ana B. Ibáñez; Celia Méndez-García; Pei-Ying Hong; Isaac Cann; Roderick I. Mackie

doi:10.1016/j.biteb.2019.100254

Identification of methanogenesis and syntrophy as important microbial metabolic processes for optimal thermophilic anaerobic digestion of energy cane thin stillage

Margreet J. Oosterkamp, Stefan Bauer, Ana B. Ibáñez, Celia Méndez-García, Pei-Ying Hong, Isaac Cann, Roderick I. Mackie

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The aim of this research was to identify key microorganisms for thermophilic (55 °C) anaerobic digestion of thin stillage derived from hydrolysis and ethanol fermentation of energy cane in a conventional stirred tank reactor with a 10-day hydraulic retention time. Efficient thermophilic anaerobic digestion with a specific methane production of 0.43 Lmethane/gtCOD used/d and biogas containing around 56% methane was accomplished. Due to an overnight temperature perturbation the specific methane production decreased to 0.16 Lmethane/gtCOD used/d. Analysis of the microbial community showed the importance of methanogenic Archaea belonging to Methanosarcina and Methanothermobacter as well as syntrophic Bacteria related to Thermacetogenium, Tepidanaerobacter and Anaerobaculum. This indicates that retention of biomass maintaining syntrophy and methanogenesis more efficiently may be useful for thermophilic anaerobic digestion of thin stillage derived from the production of energy cane ethanol.

Original language	English (US)
Pages (from-to)	100254
Journal	Bioresource Technology Reports
Volume	7
DOIs	https://doi.org/10.1016/j.biteb.2019.100254
State	Published - Jun 1 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We greatly appreciate the help of Sabrina Zimmerman (BP Biofuels, University of California at Berkeley, Berkeley, CA, USA), Glen Austin (BP pilot plant, Jennings, LA, USA), Michael Harland and Robert Brown (School of Chemical Sciences, Machine Shop, University of Illinois, Urbana, IL, USA), Chris Wright and Álvaro Hernández (W.M. Keck Center, Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL, USA). This research was supported by the Energy Biosciences Institute (USA, project OO2J14).

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@article{e92639df3ad0423789c123d90169143f,

title = "Identification of methanogenesis and syntrophy as important microbial metabolic processes for optimal thermophilic anaerobic digestion of energy cane thin stillage",

abstract = "The aim of this research was to identify key microorganisms for thermophilic (55 °C) anaerobic digestion of thin stillage derived from hydrolysis and ethanol fermentation of energy cane in a conventional stirred tank reactor with a 10-day hydraulic retention time. Efficient thermophilic anaerobic digestion with a specific methane production of 0.43 Lmethane/gtCOD used/d and biogas containing around 56% methane was accomplished. Due to an overnight temperature perturbation the specific methane production decreased to 0.16 Lmethane/gtCOD used/d. Analysis of the microbial community showed the importance of methanogenic Archaea belonging to Methanosarcina and Methanothermobacter as well as syntrophic Bacteria related to Thermacetogenium, Tepidanaerobacter and Anaerobaculum. This indicates that retention of biomass maintaining syntrophy and methanogenesis more efficiently may be useful for thermophilic anaerobic digestion of thin stillage derived from the production of energy cane ethanol.",

author = "Oosterkamp, {Margreet J.} and Stefan Bauer and Ib{\'a}{\~n}ez, {Ana B.} and Celia M{\'e}ndez-Garc{\'i}a and Pei-Ying Hong and Isaac Cann and Mackie, {Roderick I.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We greatly appreciate the help of Sabrina Zimmerman (BP Biofuels, University of California at Berkeley, Berkeley, CA, USA), Glen Austin (BP pilot plant, Jennings, LA, USA), Michael Harland and Robert Brown (School of Chemical Sciences, Machine Shop, University of Illinois, Urbana, IL, USA), Chris Wright and {\'A}lvaro Hern{\'a}ndez (W.M. Keck Center, Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL, USA). This research was supported by the Energy Biosciences Institute (USA, project OO2J14).",

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T1 - Identification of methanogenesis and syntrophy as important microbial metabolic processes for optimal thermophilic anaerobic digestion of energy cane thin stillage

AU - Oosterkamp, Margreet J.

AU - Bauer, Stefan

AU - Ibáñez, Ana B.

AU - Méndez-García, Celia

AU - Hong, Pei-Ying

AU - Cann, Isaac

AU - Mackie, Roderick I.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We greatly appreciate the help of Sabrina Zimmerman (BP Biofuels, University of California at Berkeley, Berkeley, CA, USA), Glen Austin (BP pilot plant, Jennings, LA, USA), Michael Harland and Robert Brown (School of Chemical Sciences, Machine Shop, University of Illinois, Urbana, IL, USA), Chris Wright and Álvaro Hernández (W.M. Keck Center, Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL, USA). This research was supported by the Energy Biosciences Institute (USA, project OO2J14).

PY - 2019/6/1

Y1 - 2019/6/1

N2 - The aim of this research was to identify key microorganisms for thermophilic (55 °C) anaerobic digestion of thin stillage derived from hydrolysis and ethanol fermentation of energy cane in a conventional stirred tank reactor with a 10-day hydraulic retention time. Efficient thermophilic anaerobic digestion with a specific methane production of 0.43 Lmethane/gtCOD used/d and biogas containing around 56% methane was accomplished. Due to an overnight temperature perturbation the specific methane production decreased to 0.16 Lmethane/gtCOD used/d. Analysis of the microbial community showed the importance of methanogenic Archaea belonging to Methanosarcina and Methanothermobacter as well as syntrophic Bacteria related to Thermacetogenium, Tepidanaerobacter and Anaerobaculum. This indicates that retention of biomass maintaining syntrophy and methanogenesis more efficiently may be useful for thermophilic anaerobic digestion of thin stillage derived from the production of energy cane ethanol.

AB - The aim of this research was to identify key microorganisms for thermophilic (55 °C) anaerobic digestion of thin stillage derived from hydrolysis and ethanol fermentation of energy cane in a conventional stirred tank reactor with a 10-day hydraulic retention time. Efficient thermophilic anaerobic digestion with a specific methane production of 0.43 Lmethane/gtCOD used/d and biogas containing around 56% methane was accomplished. Due to an overnight temperature perturbation the specific methane production decreased to 0.16 Lmethane/gtCOD used/d. Analysis of the microbial community showed the importance of methanogenic Archaea belonging to Methanosarcina and Methanothermobacter as well as syntrophic Bacteria related to Thermacetogenium, Tepidanaerobacter and Anaerobaculum. This indicates that retention of biomass maintaining syntrophy and methanogenesis more efficiently may be useful for thermophilic anaerobic digestion of thin stillage derived from the production of energy cane ethanol.

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ER -

Identification of methanogenesis and syntrophy as important microbial metabolic processes for optimal thermophilic anaerobic digestion of energy cane thin stillage

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