Identification of a ribonuclease H gene in both Mycoplasma genitalium and Mycoplasma pneumoniae by a new method for exhaustive identification of ORFs in the complete genome sequences

Matthew I. Bellgard; Takashi Gojobori

doi:10.1016/S0014-5793(99)00075-7

Identification of a ribonuclease H gene in both Mycoplasma genitalium and Mycoplasma pneumoniae by a new method for exhaustive identification of ORFs in the complete genome sequences

Matthew I. Bellgard^*, Takashi Gojobori

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Exhaustive identification of open reading frames in complete genome sequences is a difficult task. It is possible that important genes are missed. In our efforts to reanalyze the intergenic regions of Mycoplasma genitalium and Mycoplasma pneumoniae, we have newly identified a number of new open reading frames (ORFs) in both M. genitalium and M. pneumoniae. The most significant identification was that of a ribonuclease H enzyme in both species which until now has not been identified or assumed absent and interpreted as such. In this paper we discuss the biological importance of RNase H and its evolutionary implication. We also stress the usefulness of our method for identifying new ORFs by reanalyzing intergenic regions of existing ORFs in complete genome sequences. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	6-8
Number of pages	3
Journal	FEBS Letters
Volume	445
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(99)00075-7
State	Published - Feb 19 1999
Externally published	Yes

Bibliographical note

Funding Information:
We thank Dr. Naoko Takezaki for her advice on the phylogenetic analysis. This research was made possible by a postdoctoral fellowship from the Japan Society for the Promotion of Science in Australia.

Keywords

Complete genome sequence
Mycoplasma genitalium
Mycoplasma pneumoniae
Open reading frame
Ribonuclease H

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)00075-7

Cite this

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title = "Identification of a ribonuclease H gene in both Mycoplasma genitalium and Mycoplasma pneumoniae by a new method for exhaustive identification of ORFs in the complete genome sequences",

abstract = "Exhaustive identification of open reading frames in complete genome sequences is a difficult task. It is possible that important genes are missed. In our efforts to reanalyze the intergenic regions of Mycoplasma genitalium and Mycoplasma pneumoniae, we have newly identified a number of new open reading frames (ORFs) in both M. genitalium and M. pneumoniae. The most significant identification was that of a ribonuclease H enzyme in both species which until now has not been identified or assumed absent and interpreted as such. In this paper we discuss the biological importance of RNase H and its evolutionary implication. We also stress the usefulness of our method for identifying new ORFs by reanalyzing intergenic regions of existing ORFs in complete genome sequences. Copyright (C) 1999 Federation of European Biochemical Societies.",

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author = "Bellgard, {Matthew I.} and Takashi Gojobori",

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AU - Gojobori, Takashi

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KW - Complete genome sequence

KW - Mycoplasma genitalium

KW - Mycoplasma pneumoniae

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Identification of a ribonuclease H gene in both Mycoplasma genitalium and Mycoplasma pneumoniae by a new method for exhaustive identification of ORFs in the complete genome sequences

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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