Identification and experimental characterization of an extremophilic brine pool alcohol dehydrogenase from single amplified genomes

Stefan W. Grötzinger, Ram Karan, Eva Strillinger, Stefan Bader, Annika Frank, Israa Al Rowaihi, Anastassja Gespers (Akal), Wiebke Wackerow, John A.C. Archer, Magnus Rueping, Dirk Weuster-Botz, Michael Groll, Jörg Eppinger, Stefan T. Arold

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Because only 0.01% of prokaryotic genospecies can be cultured and in situ observations are often impracticable, culture-independent methods are required to understand microbial life and harness potential applications of microbes. Here, we report a methodology for the production of proteins with desired functions based on single amplified genomes (SAGs) from unculturable species. We use this method to resurrect an alcohol dehydrogenase (ADH/D1) from an uncharacterized halo-thermophilic archaeon collected from a brine pool at the bottom of the Red Sea. Our crystal structure of 5,6-dihydroxy NADPH-bound ADH/D1 combined with biochemical analyses reveal the molecular features of its halo-thermophily, its unique habitat adaptations, and its possible reaction mechanism for atypical oxygen activation. Our strategy offers a general guide for using SAGs as a source for scientific and industrial investigations of ‘microbial dark matter’.
Original languageEnglish (US)
Pages (from-to)161-170
Number of pages10
JournalACS Chemical Biology
Issue number1
StatePublished - Dec 18 2017

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): URF/1/1976.06, URF/1/2602.01.01, URF/1/1974
Acknowledgements: The research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST) through the baseline fund and the Office of Sponsored Research (OSR) under award nos. URF/1/1976.06, URF/1/2602.01.01, and URF/1/1974. The authors are thankful for support for E.S. and S.G. from the International Graduate School of Science and Engineering (IGGSE), Technical University of Munich (TUM), project 8.03.


Dive into the research topics of 'Identification and experimental characterization of an extremophilic brine pool alcohol dehydrogenase from single amplified genomes'. Together they form a unique fingerprint.

Cite this