Abstract
Hydroxyurea (HU), as a therapeutic medicine, has been extensively used clinically. To further survey molecular mechanisms of HU treatment, we analyzed global transcriptomic alteration of mouse ES cells in response to the treatment using high-throughput sequencing. We show that the global transcriptional activity is significantly suppressed as cells are exposed to HU treatment and alters multiple key cellular pathways, including cell cycle, apoptosis and DNAs. HU treatment also alters alternative splicing mechanisms and suppresses non-coding RNA expression. Our result provides novel clues for the understanding of how cells respond to HU and further suggests that high-throughput sequencing technology provides a powerful tool to study mechanisms of clinical drugs at the cellular level. Published by Oxford University Press 2010.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1661-1668 |
| Number of pages | 8 |
| Journal | Carcinogenesis |
| Volume | 31 |
| Issue number | 9 |
| DOIs | |
| State | Published - May 31 2010 |
Bibliographical note
Funding Information:National Basic Research Program (973 Program) (2006CB910401, 2006CB910403 and 2006CB910404 to J.Y. and S.H.); National Protein Project of Ministry of Science and Technology (2006CB910902 to H.H.); Ministry of Science and Technology of the People’s Republic of China; Knowledge Innovation Program of Chinese Academy of Sciences (KSCX2-YW-R63 and KJCX2-YW-L08 to H.H.); National Natural Science Foundation of China (30530180 to H.H.).
ASJC Scopus subject areas
- Cancer Research
