HvNax3 - A locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum)

Yuri Shavrukov; Narendra K. Gupta; Junji Miyazaki; Manahil N. Baho; Kenneth J. Chalmers; Mark Tester; Peter Langridge; Nicholas C. Collins

doi:10.1007/s10142-009-0153-8

HvNax3 - A locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum)

Yuri Shavrukov^*, Narendra K. Gupta, Junji Miyazaki, Manahil N. Baho, Kenneth J. Chalmers, Mark Tester, Peter Langridge, Nicholas C. Collins

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

125 Scopus citations

Abstract

Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na⁺) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection of the cultivated barley (H. vulgare ssp. vulgare) cultivar Barque (Barque-73, a moderate Na⁺ excluder) attributed the control of the Na⁺ exclusion trait from CPI-71284-48 to a single locus on the short arm of chromosome 7H, which was named HvNax3. The locus reduced shoot Na ⁺ accumulation by 10-25% in plants grown in 150 mM NaCl. Markers generated using colinearity with rice and Brachypodium, together with the analysis of introgression lines and F2 and F3 families, enabled HvNax3 to be mapped to a 1.3-cM interval. Genes from the corresponding rice and Brachypodium intervals encode 16 different classes of proteins and include several plausible candidates for HvNax3. The potential of HvNax3 to provide a useful trait contributing to salinity tolerance in cultivated barley is discussed.

Original language	English (US)
Pages (from-to)	277-291
Number of pages	15
Journal	Functional and Integrative Genomics
Volume	10
Issue number	2
DOIs	https://doi.org/10.1007/s10142-009-0153-8
State	Published - May 2010
Externally published	Yes

Keywords

Barley
H. vulgare ssp. Spontaneum
Pyrophosphatase
Salinity tolerance
Sodium transport

ASJC Scopus subject areas

Genetics

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10.1007/s10142-009-0153-8

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title = "HvNax3 - A locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum)",

abstract = "Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na+) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection of the cultivated barley (H. vulgare ssp. vulgare) cultivar Barque (Barque-73, a moderate Na+ excluder) attributed the control of the Na+ exclusion trait from CPI-71284-48 to a single locus on the short arm of chromosome 7H, which was named HvNax3. The locus reduced shoot Na + accumulation by 10-25% in plants grown in 150 mM NaCl. Markers generated using colinearity with rice and Brachypodium, together with the analysis of introgression lines and F2 and F3 families, enabled HvNax3 to be mapped to a 1.3-cM interval. Genes from the corresponding rice and Brachypodium intervals encode 16 different classes of proteins and include several plausible candidates for HvNax3. The potential of HvNax3 to provide a useful trait contributing to salinity tolerance in cultivated barley is discussed.",

keywords = "Barley, H. vulgare ssp. Spontaneum, Pyrophosphatase, Salinity tolerance, Sodium transport",

author = "Yuri Shavrukov and Gupta, {Narendra K.} and Junji Miyazaki and Baho, {Manahil N.} and Chalmers, {Kenneth J.} and Mark Tester and Peter Langridge and Collins, {Nicholas C.}",

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AU - Shavrukov, Yuri

AU - Gupta, Narendra K.

AU - Miyazaki, Junji

AU - Baho, Manahil N.

AU - Chalmers, Kenneth J.

AU - Tester, Mark

AU - Langridge, Peter

AU - Collins, Nicholas C.

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HvNax3 - A locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum)

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