Abstract
Scope: To analyse the usefulness of isolated human peripheral blood mononuclear cells (PBMC) to rapidly/easily reflect n-3 long-chain polyunsaturated fatty acid (LCPUFA) effects on lipid metabolism/inflammation gene profile, and evaluate if these effects are body mass index (BMI) dependent. Methods and results: PBMC from normoweight (NW) and overweight/obese (OW/OB) subjects were incubated with physiological doses of docosahexaenoic (DHA), eicosapentaenoic acid (EPA), or their combination. PBMC reflected increased beta-oxidation-like capacity (CPT1A expression) in OW/OB but only after DHA treatment. However, insensitivity to n-3 LCPUFA was evident in OW/OB for lipogenic genes: both PUFA diminished FASN and SREBP1C expression in NW, but no effect was observed for DHA in PBMC from high-BMI subjects. This insensitivity was also evident for inflammation gene profile: all treatments inhibited key inflammatory genes in NW; nevertheless, no effect was observed in OW/OB after DHA treatment, and EPA effect was impaired. SLC27A2, IL6 and TNFα PBMC expression analysis resulted especially interesting to determine obesity-related n-3 LCPUFA insensitivity. Conclusion: A PBMC-based human in vitro system reflects n-3 LCPUFA effects on lipid metabolism/inflammation which is impaired in OW/OB. These results confirm the utility of PBMC ex vivo systems for bioactive-compound screening to promote functional food development and to establish appropriate dietary strategies for obese population.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1600353 |
| Journal | Molecular Nutrition & Food Research |
| Volume | 61 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 30 2016 |
Bibliographical note
KAUST Repository Item: Exported on 2020-10-01Acknowledgements: A.P and P.O conceived the experiment. M.C did the major part of the experimental work. R.D-R, R.V-C, B.R and J.P-B were involved in the experimental set-up, experimental work and sample collection. M.C and P.O wrote the article; all the authors participated in critical revision of the manuscript. We thank Enzo Ceresi for technical assistance in the determination of PBMC cell populations. CIBER de Fisiopatología de la Obesidad y Nutrición is an initiative of the ISCIII. This work was supported by the Spanish Government (Ministerio de Economía y Competititividad, INTERBIOBES -AGL2015-67019-P- and EPIMILK -AGL2012-33692-) and by the University of the Balearic Islands (IBIFLEX, FA38/2016). The Laboratory of Molecular Biology, Nutrition and Biotechnology is member of the European Research Network of Excellence NuGO (The European Nutrigenomics Organization, EU Contract: FOOD-CT-2004-506360 NUGO). MC is a recipient of a fellowship from the Spanish Government.
