CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a given application among thousands of potential candidates. We provide an overview of the currently described Cas effector systems and review existing Cas-based RNA detection methods. We then propose a set of systematic selection criteria for selecting CRISPR-Cas candidates for new applications. Using this approach, we identify four candidates for in vitro RNA.
Bibliographical noteKAUST Repository Item: Exported on 2022-01-25
Acknowledged KAUST grant number(s): OSR, REI/1/4204-01
Acknowledgements: This research was supported by the King Abdullah University of Science and Technology (KAUST) through the baseline fund and Award No. REI/1/4204-01 from the Office of Sponsored Research (OSR).
ASJC Scopus subject areas
- Clinical Biochemistry