How to Find the Right RNA-Sensing CRISPR-Cas System for an In Vitro Application

Escarlet Díaz-Galicia; Raik Grunberg; Stefan T. Arold

doi:10.3390/bios12020053

How to Find the Right RNA-Sensing CRISPR-Cas System for an In Vitro Application

Escarlet Díaz-Galicia, Raik Grunberg, Stefan T. Arold

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a given application among thousands of potential candidates. We provide an overview of the currently described Cas effector systems and review existing Cas-based RNA detection methods. We then propose a set of systematic selection criteria for selecting CRISPR-Cas candidates for new applications. Using this approach, we identify four candidates for in vitro RNA.

Original language	English (US)
Pages (from-to)	53
Journal	Biosensors
Volume	12
Issue number	2
DOIs	https://doi.org/10.3390/bios12020053
State	Published - Jan 19 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-01-25
Acknowledged KAUST grant number(s): OSR, REI/1/4204-01
Acknowledgements: This research was supported by the King Abdullah University of Science and Technology (KAUST) through the baseline fund and Award No. REI/1/4204-01 from the Office of Sponsored Research (OSR).

ASJC Scopus subject areas

Clinical Biochemistry

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10.3390/bios12020053

https://repository.kaust.edu.sa/bitstream/10754/675108/1/biosensors-12-00053.pdf

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title = "How to Find the Right RNA-Sensing CRISPR-Cas System for an In Vitro Application",

abstract = "CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a given application among thousands of potential candidates. We provide an overview of the currently described Cas effector systems and review existing Cas-based RNA detection methods. We then propose a set of systematic selection criteria for selecting CRISPR-Cas candidates for new applications. Using this approach, we identify four candidates for in vitro RNA.",

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N2 - CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a given application among thousands of potential candidates. We provide an overview of the currently described Cas effector systems and review existing Cas-based RNA detection methods. We then propose a set of systematic selection criteria for selecting CRISPR-Cas candidates for new applications. Using this approach, we identify four candidates for in vitro RNA.

AB - CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a given application among thousands of potential candidates. We provide an overview of the currently described Cas effector systems and review existing Cas-based RNA detection methods. We then propose a set of systematic selection criteria for selecting CRISPR-Cas candidates for new applications. Using this approach, we identify four candidates for in vitro RNA.

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How to Find the Right RNA-Sensing CRISPR-Cas System for an In Vitro Application

Abstract

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