High throughput second harmonic imaging for label-free biological applications

Carlos Macias Romero; Marie E P Didier; Pascal Jourdain; Pierre Marquet; Pierre J. Magistretti; Orly B. Tarun; Vitalijs Zubkovs; Aleksandra Radenovic; Sylvie Roke

doi:10.1364/OE.22.031102

High throughput second harmonic imaging for label-free biological applications

Carlos Macias Romero, Marie E P Didier, Pascal Jourdain, Pierre Marquet, Pierre J. Magistretti, Orly B. Tarun, Vitalijs Zubkovs, Aleksandra Radenovic, Sylvie Roke

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.

Original language	English (US)
Pages (from-to)	31102
Journal	Optics Express
Volume	22
Issue number	25
DOIs	https://doi.org/10.1364/OE.22.031102
State	Published - Dec 8 2014

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the Julia Jacobi Foundation, the Swiss National Foundation (grant number 200021-146884), and European Commission, Research Executive Agency Marie Curie Actions 'FINON' (ITN-2013-607842). We would like to thank Prof. K. B. Eisenthal for very inspiring discussions.

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OE.22.031102

Cite this

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title = "High throughput second harmonic imaging for label-free biological applications",

abstract = "Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.",

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note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the Julia Jacobi Foundation, the Swiss National Foundation (grant number 200021-146884), and European Commission, Research Executive Agency Marie Curie Actions 'FINON' (ITN-2013-607842). We would like to thank Prof. K. B. Eisenthal for very inspiring discussions.",

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doi = "10.1364/OE.22.031102",

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AU - Macias Romero, Carlos

AU - Didier, Marie E P

AU - Jourdain, Pascal

AU - Marquet, Pierre

AU - Magistretti, Pierre J.

AU - Tarun, Orly B.

AU - Zubkovs, Vitalijs

AU - Radenovic, Aleksandra

AU - Roke, Sylvie

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the Julia Jacobi Foundation, the Swiss National Foundation (grant number 200021-146884), and European Commission, Research Executive Agency Marie Curie Actions 'FINON' (ITN-2013-607842). We would like to thank Prof. K. B. Eisenthal for very inspiring discussions.

PY - 2014/12/8

Y1 - 2014/12/8

N2 - Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.

AB - Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.

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High throughput second harmonic imaging for label-free biological applications

Abstract

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