In this study, both PtSn/C electrocatalysts and composites electrolytes based on Nafion and mesoporous titania (Nafion-TiO2) were evaluated in direct ethanol fuel cell operating at 130°C. PtSn/C catalysts with different compositions were prepared by alcohol-reduction process. The electrochemical activity of these electrocatalysts was verified by cyclic voltammetry and chronoamperometry measurements. Cast Nafion-TiO2 composites were characterized by differential scanning calorimetry and ac impedance spectroscopy. The water uptake of the composites was determined by gravimetry. Membrane electrode assemblies composed by Nafion-TiO2 electrolyte and PtSn/C anode were evaluate in DEFC operating in 80-130°C temperature range. Polarization curves showed an enhanced performance (maximum power density of ∼50 mW cm-2) for DEFC using combined PtSn/C (60:40) electrocatalyst and Nafion-TiO2 (10 wt%) operating at 130°C.