High-resolution phenotypic profiling of natural products-induced effects on the single-cell level

Stephan Georg Kremb; Christian R. Voolstra

doi:10.1038/srep44472

High-resolution phenotypic profiling of natural products-induced effects on the single-cell level

Stephan Georg Kremb, Christian R. Voolstra

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.

Original language	English (US)
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/srep44472
State	Published - Mar 15 2017

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We thank the Bioscience core lab for supporting cell culture work and providing the Cellomics ArrayScan HCS instrument. Research in this publication was supported by baseline funds to CRV from King Abdullah University of Science and Technology (KAUST).

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10.1038/srep44472

https://repository.kaust.edu.sa/bitstream/10754/623036/1/srep44472.pdf

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title = "High-resolution phenotypic profiling of natural products-induced effects on the single-cell level",

abstract = "Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.",

author = "Kremb, {Stephan Georg} and Voolstra, {Christian R.}",

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AB - Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.

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High-resolution phenotypic profiling of natural products-induced effects on the single-cell level

Abstract

Bibliographical note

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