Hierarchical hybrid peroxidase catalysts for remediation of phenol wastewater

Xiaonan Duan, Stéphane C. Corgié, Daniel J. Aneshansley, Peng Wang, Larry P. Walker, Emmanuel P. Giannelis

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


We report a new family of hierarchical hybrid catalysts comprised of horseradish peroxidase (HRP)-magnetic nanoparticles for advanced oxidation processes and demonstrate their utility in the removal of phenol from water. The immobilized HRP catalyzes the oxidation of phenols in the presence of H2O2, producing free radicals. The phenoxy radicals react with each other in a non-enzymatic process to form polymers, which can be removed by precipitation with salts or condensation. The hybrid peroxidase catalysts exhibit three times higher activity than free HRP and are able to remove three times more phenol from water compared to free HRP under similar conditions. In addition, the hybrid catalysts reduce substrate inhibition and limit inactivation from reaction products, which are common problems with free or conventionally immobilized enzymes. Reusability is improved when the HRP-magnetic nanoparticle hybrids are supported on micron-scale magnetic particles, and can be retained with a specially designed magnetically driven reactor. The performance of the hybrid catalysts makes them attractive for several industrial and environmental applications and their development might pave the way for practical applications by eliminating most of the limitations that have prevented the use of free or conventionally immobilized enzymes. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish (US)
Pages (from-to)974-980
Number of pages7
Issue number5
StatePublished - Feb 20 2014

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): KUS-C1-018-02
Acknowledgements: This publication was based on work supported by Award No. KUS-C1-018-02, made by the King Abdullah University of Science and Technology (KAUST). We acknowledge use of the Biofuel Research Laboratory (BRL) at Cornell University and the Cornell Center for Materials Research Shared Facilities, which are supported through the National Science Foundation Materials Research Science and Engineering Centers (NSF MRSEC) (DMR-1120296). The magnetically driven reactor part was supported by the US Department of Transportation under contract to the Northeast Sun Grant Initiative at Cornell University #US DOT Assistance #DTOS59-07-G-00052. The authors thank Yan Kang for his help with the SEM images and Dr. Panagiotis Dallas for discussions.

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry


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