TY - JOUR
T1 - Hematologic reference intervals and age effect in European Strigiformes
AU - Agusti Montolio, Susana
AU - Molina López, Rafael
AU - Cray, Carolyn
AU - Lavín González, Santiago
AU - Nicolás Francisco, Olga
AU - Marco Sánchez, Ignasi
AU - Casas-Díaz, Encarna
AU - Cuenca Valera, Rafaela
N1 - Publisher Copyright:
© 2017 American Society for Veterinary Clinical Pathology
PY - 2017/9
Y1 - 2017/9
N2 - Background: The clinical importance of hematologic testing in avian veterinary medicine is reflected in the increasing number of studies for the establishment of hematologic RIs of Strigiformes and other species. Age is an important physiologic factor in birds and the effect on hematology variable should be understood. Objectives: The objective of this study was to determine baseline data of hematologic variables in 5 species of Iberian Strigiformes in different age classes. Methods: Nocturnal birds of prey were sampled at Wildlife Health Centers. Packed cell volume was determined by the microhematocrit centrifugation method, and RBC and WBC counts were determined using the direct hemocytometer count method with Natt and Herrick solution. Hemoglobin concentration was measured spectrophotometrically. The MCV, MCHC, and MHC were calculated using the standard formulas. The differential WBC count was performed by the routine microscopic evaluation of 200 cells on a blood smear manually stained with Wright stain. Thrombocyte blood count estimate was obtained from the blood film. Results: No differences were observed between juveniles and adults for any variable evaluated in Tawny owl, Little owl, Scops owl, Long-eared owl, and Barn owl. In addition, PCV, RBC, and HGB of chicks were statistically significantly lower than in juveniles and adults, and total WBC was significantly higher in Tawny owl, Little owl, Scops owl, and Long-eared owl. Conclusions: Our findings provide evidence that laboratory data from chicks of Strigiformes are different compared to juveniles and adults; therefore, separate RIs were defined.
AB - Background: The clinical importance of hematologic testing in avian veterinary medicine is reflected in the increasing number of studies for the establishment of hematologic RIs of Strigiformes and other species. Age is an important physiologic factor in birds and the effect on hematology variable should be understood. Objectives: The objective of this study was to determine baseline data of hematologic variables in 5 species of Iberian Strigiformes in different age classes. Methods: Nocturnal birds of prey were sampled at Wildlife Health Centers. Packed cell volume was determined by the microhematocrit centrifugation method, and RBC and WBC counts were determined using the direct hemocytometer count method with Natt and Herrick solution. Hemoglobin concentration was measured spectrophotometrically. The MCV, MCHC, and MHC were calculated using the standard formulas. The differential WBC count was performed by the routine microscopic evaluation of 200 cells on a blood smear manually stained with Wright stain. Thrombocyte blood count estimate was obtained from the blood film. Results: No differences were observed between juveniles and adults for any variable evaluated in Tawny owl, Little owl, Scops owl, Long-eared owl, and Barn owl. In addition, PCV, RBC, and HGB of chicks were statistically significantly lower than in juveniles and adults, and total WBC was significantly higher in Tawny owl, Little owl, Scops owl, and Long-eared owl. Conclusions: Our findings provide evidence that laboratory data from chicks of Strigiformes are different compared to juveniles and adults; therefore, separate RIs were defined.
KW - Blood
KW - owls
KW - values
UR - http://www.scopus.com/inward/record.url?scp=85023771140&partnerID=8YFLogxK
U2 - 10.1111/vcp.12516
DO - 10.1111/vcp.12516
M3 - Article
C2 - 28712138
AN - SCOPUS:85023771140
SN - 0275-6382
VL - 46
SP - 483
EP - 495
JO - Veterinary Clinical Pathology
JF - Veterinary Clinical Pathology
IS - 3
ER -