The wide number of industrial processes applying cellulases highlights the importance of discovering robust enzymes able to work under harsh conditions. In this study, carboxymethyl cellulase (CMCase) activity of Paenibacillus tarimensis was characterized. A high activity was observed in pH range 3.0-10.5 and 9. mM-5. M NaCl. In high salt buffer at 80. °C, >80% and >76% of relative activity was retained at 20% of the ionic liquids (ILs) [EMIM]Ac and [BMIM]Cl; while >40% was detected with 40% [BMIM]Cl. Five CMCases were detected by renaturing SDS-PAGE. Their activity was retained in presence of 1.7 up to 5. M NaCl (for CMC1) or 4.6. M KCl; 5% organic solvents or 10. mM bivalent ions, EDTA and heavy metals; under neutral and halo-alkaline conditions. These cellulases stabile and highly functional under harsh conditions are promising candidates for application in detergents, textiles, paper/pulp industry; and simultaneous ILs treatment-saccharification of lignocellulose.
Bibliographical noteFunding Information:
The support of the Italian Ministero per le Politiche Agricole, Alimentari e Forestali (MiPAAF) (project EXTRAVALORE) and the FP7 EU project BIODESERT ( GA-245746 ) is acknowledged.
- Ionic liquids
- Paenibacillus tarimensis
- Poly-extremotolerant cellulases
ASJC Scopus subject areas
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal