Halo-alkalitolerant and thermostable cellulases with improved tolerance to ionic liquids and organic solvents from Paenibacillus tarimensis isolated from the Chott El Fejej, Sahara desert, Tunisia

Noura Raddadi*, Ameur Cherif, Daniele Daffonchio, Fabio Fava

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


The wide number of industrial processes applying cellulases highlights the importance of discovering robust enzymes able to work under harsh conditions. In this study, carboxymethyl cellulase (CMCase) activity of Paenibacillus tarimensis was characterized. A high activity was observed in pH range 3.0-10.5 and 9. mM-5. M NaCl. In high salt buffer at 80. °C, >80% and >76% of relative activity was retained at 20% of the ionic liquids (ILs) [EMIM]Ac and [BMIM]Cl; while >40% was detected with 40% [BMIM]Cl. Five CMCases were detected by renaturing SDS-PAGE. Their activity was retained in presence of 1.7 up to 5. M NaCl (for CMC1) or 4.6. M KCl; 5% organic solvents or 10. mM bivalent ions, EDTA and heavy metals; under neutral and halo-alkaline conditions. These cellulases stabile and highly functional under harsh conditions are promising candidates for application in detergents, textiles, paper/pulp industry; and simultaneous ILs treatment-saccharification of lignocellulose.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalBioresource Technology
StatePublished - Jan 1 2013


  • Chott
  • Ionic liquids
  • Paenibacillus tarimensis
  • Poly-extremotolerant cellulases

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

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