In hepatic cancer, precancerous nodules account for damage and inflammation in liver cells. Studies have proved that phyto-compounds based on biosynthetic metallic nanoparticles display superior action against hepatic tumors. This study targeted the synthesis of genistein-fortified zinc ferrite nanoparticles (GENP) trailed by anticancer activity assessment against diethylnitrosamine and N-acetyl-2-aminofluorene induced hepatic cancer. The process of nucleation was confirmed by UV-Vis spectrophotometry, X-ray beam diffraction, field-emission scanning electron microscopy, and FTIR. An in vitro antioxidant assay illustrated that the leaves of Pterocarpus mildbraedii have strong tendency as a reducetant and, in the nanoformulation synthesis, as a natural capping agent. A MTT assay confirmed that GENP have a strong selective cytotoxic potential against HepG2 cancer cells. In silico studies of genistein exemplified the binding tendency towards human matrix metalloproteinase comparative to the standard drug marimastat. An in vivo anticancer evaluation showed that GENP effectively inhibit the growth of hepatic cancer by interfering with hepatic and non-hepatic biochemical markers.
Bibliographical noteKAUST Repository Item: Exported on 2023-06-19
Acknowledgements: The authors acknowledge the Alliance for Accelerating Excellence in Science in Africa (AESA) and partners, the Welcome Trust/DBT India Alliance, and the African Academy of Sciences (AAS), for Grant AIMF-20-002 to conduct this study. CAO acknowledges the Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj for technical and administrative support in the course of this study. JN thanks the Univer-sity of Zagreb, University Computing Centre — SRCE, for granting access to the Isabella computer cluster, and the University of Rijeka for support through the Uniri-mladi-prirod-22-8 grant. PP and MG acknowledged South Ural State University, Chelyabinsk, Russia under Priority 2030 program.
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