Gold nanostructured laser-scribed graphene: A new electrochemical biosensing platform for potential point-of-care testing of disease biomarkers

Sakandar Rauf, Abdellatif Ait Lahcen, Abdulrahman Aljedaibi, Tutku Beduk, José Ilton de Oliveira Filho, Khaled N. Salama

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Improvements in the Laser-scribed graphene (LSG)-based electrodes are critical to overcoming limitations of bare LSG electrodes in terms of sensitivity, direct immobilization of detection probes for biosensor fabrication, and ease of integration with point-of-care (POC) devices. Herein, we introduce a new class of nanostructured gold modified LSG (LSG-AuNS) electrochemical sensing system comprising LSG-AuNS working electrode, LSG reference, and LSG counter electrode. LSG-AuNS electrodes are realized by electrodeposition of gold chloride (HAuCl4) solution, which gave ∼2-fold enhancement in sensitivity and electrocatalytic activity compared to bare LSG electrode and commercially available screen-printed gold electrode (SPAuE). We demonstrate LSG-AuNS electrochemical aptasensor for detecting human epidermal growth factor receptor 2 (Her-2) with a limit of detection (LOD) of 0.008 ng/mL and a linear range of 0.1-200 ng/mL. LSG-AuNS-aptasensor can easily detect different concentrations of Her-2 spiked in undiluted human serum. Finally, to show the LSG-AuNS sensor system's potential to develop POC biosensor devices, we integrated LSG-AuNS electrodes with a handheld electrochemical system operated using a custom-developed mobile application.
Original languageEnglish (US)
Pages (from-to)113116
JournalBiosensors and Bioelectronics
DOIs
StatePublished - Feb 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-03-01
Acknowledgements: The authors would like to express their acknowledgements to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative and KAUST visiting student program for supporting this work.

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