Glycogen metabolism as a marker of astrocyte differentiation

J. F. Brunet; I. Allaman; P. J. Magistretti; L. Pellerin

doi:10.1038/jcbfm.2009.207

Glycogen metabolism as a marker of astrocyte differentiation

J. F. Brunet, I. Allaman, P. J. Magistretti, L. Pellerin

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Glycogen is a hallmark of mature astrocytes, but its emergence during astrocytic differentiation is unclear. Differentiation of E14 mouse neurospheres into astrocytes was induced with fetal bovine serum (FBS), Leukemia Inhibitory Factor (LIF), or Ciliary Neurotrophic Factor (CNTF). Cytochemical and enzymatic analyses showed that glycogen is present in FBS-or LIF-but not in CNTF-differentiated astrocytes. Glycogenolysis was induced in FBS-and LIF-differentiated astrocytes but glycogen resynthesis was observed only with FBS. Protein targeting to glycogen mRNA expression appeared with glial fibrillary acidic protein and S100Β in FBS and LIF conditions but not with CNTF. These results show that glycogen metabolism constitutes a useful marker of astrocyte differentiation.

Original language	English (US)
Pages (from-to)	51-55
Number of pages	5
Journal	Journal of Cerebral Blood Flow and Metabolism
Volume	30
Issue number	1
DOIs	https://doi.org/10.1038/jcbfm.2009.207
State	Published - Jan 2010
Externally published	Yes

Keywords

Astrocyte differentiation
Brain energy metabolism
Glycogen
Leukemia Inhibitory Factor

ASJC Scopus subject areas

Neurology
Clinical Neurology
Cardiology and Cardiovascular Medicine

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10.1038/jcbfm.2009.207

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abstract = "Glycogen is a hallmark of mature astrocytes, but its emergence during astrocytic differentiation is unclear. Differentiation of E14 mouse neurospheres into astrocytes was induced with fetal bovine serum (FBS), Leukemia Inhibitory Factor (LIF), or Ciliary Neurotrophic Factor (CNTF). Cytochemical and enzymatic analyses showed that glycogen is present in FBS-or LIF-but not in CNTF-differentiated astrocytes. Glycogenolysis was induced in FBS-and LIF-differentiated astrocytes but glycogen resynthesis was observed only with FBS. Protein targeting to glycogen mRNA expression appeared with glial fibrillary acidic protein and S100Β in FBS and LIF conditions but not with CNTF. These results show that glycogen metabolism constitutes a useful marker of astrocyte differentiation.",

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AU - Brunet, J. F.

AU - Allaman, I.

AU - Magistretti, P. J.

AU - Pellerin, L.

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AB - Glycogen is a hallmark of mature astrocytes, but its emergence during astrocytic differentiation is unclear. Differentiation of E14 mouse neurospheres into astrocytes was induced with fetal bovine serum (FBS), Leukemia Inhibitory Factor (LIF), or Ciliary Neurotrophic Factor (CNTF). Cytochemical and enzymatic analyses showed that glycogen is present in FBS-or LIF-but not in CNTF-differentiated astrocytes. Glycogenolysis was induced in FBS-and LIF-differentiated astrocytes but glycogen resynthesis was observed only with FBS. Protein targeting to glycogen mRNA expression appeared with glial fibrillary acidic protein and S100Β in FBS and LIF conditions but not with CNTF. These results show that glycogen metabolism constitutes a useful marker of astrocyte differentiation.

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KW - Glycogen

KW - Leukemia Inhibitory Factor

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Glycogen metabolism as a marker of astrocyte differentiation

Abstract

Keywords

ASJC Scopus subject areas

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