TY - JOUR
T1 - Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes
AU - Devasenathipathy, Rajkumar
AU - Mani, Veerappan
AU - Chen, Shen Ming
AU - Huang, Sheng Tung
AU - Huang, Tsung Tao
AU - Lin, Chun Mao
AU - Hwa, Kuo Yuan
AU - Chen, Ting Yo
AU - Chen, Bo Jun
N1 - Generated from Scopus record by KAUST IRTS on 2023-09-21
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5×10-10molcm-2 and 3.36s-1, respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10μM-2mM with LOD of 4.1μM, (2) 2mM-5.2mM with LOD of 0.95mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible.
AB - Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5×10-10molcm-2 and 3.36s-1, respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10μM-2mM with LOD of 4.1μM, (2) 2mM-5.2mM with LOD of 0.95mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible.
UR - https://linkinghub.elsevier.com/retrieve/pii/S0141022915300120
UR - http://www.scopus.com/inward/record.url?scp=84932620378&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2015.06.006
DO - 10.1016/j.enzmictec.2015.06.006
M3 - Article
SN - 0141-0229
VL - 78
SP - 40
EP - 45
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
ER -