Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes

Rajkumar Devasenathipathy, Veerappan Mani, Shen Ming Chen, Sheng Tung Huang, Tsung Tao Huang, Chun Mao Lin, Kuo Yuan Hwa, Ting Yo Chen, Bo Jun Chen

Research output: Contribution to journalArticlepeer-review

115 Scopus citations


Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5×10-10molcm-2 and 3.36s-1, respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10μM-2mM with LOD of 4.1μM, (2) 2mM-5.2mM with LOD of 0.95mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible.
Original languageEnglish (US)
Pages (from-to)40-45
Number of pages6
JournalEnzyme and Microbial Technology
StatePublished - Oct 1 2015
Externally publishedYes

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