Genotypic diversity, antibiotic resistance and bacteriocin production of enterococci isolated from rhizospheres

Naouel Klibi; Naouel Ben Slimen; Imen Fhoula; Maria López; Karim Ben Slama; Daniele Daffonchio; Abdellatif Boudabous; Carmen Torres; Hadda Ouzari

doi:10.1264/jsme2.ME12041

Genotypic diversity, antibiotic resistance and bacteriocin production of enterococci isolated from rhizospheres

Naouel Klibi, Naouel Ben Slimen, Imen Fhoula, Maria López, Karim Ben Slama, Daniele Daffonchio, Abdellatif Boudabous, Carmen Torres, Hadda Ouzari^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615.

Original language	English (US)
Pages (from-to)	533-537
Number of pages	5
Journal	Microbes and Environments
Volume	27
Issue number	4
DOIs	https://doi.org/10.1264/jsme2.ME12041
State	Published - Dec 2012
Externally published	Yes

Keywords

Antibiotic resistance
Bacteriocins
Enterococci
MLST
PFGE

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

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10.1264/jsme2.ME12041

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title = "Genotypic diversity, antibiotic resistance and bacteriocin production of enterococci isolated from rhizospheres",

abstract = "This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615.",

keywords = "Antibiotic resistance, Bacteriocins, Enterococci, MLST, PFGE",

author = "Naouel Klibi and {Ben Slimen}, Naouel and Imen Fhoula and Maria L{\'o}pez and {Ben Slama}, Karim and Daniele Daffonchio and Abdellatif Boudabous and Carmen Torres and Hadda Ouzari",

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AU - Klibi, Naouel

AU - Ben Slimen, Naouel

AU - Fhoula, Imen

AU - López, Maria

AU - Ben Slama, Karim

AU - Daffonchio, Daniele

AU - Boudabous, Abdellatif

AU - Torres, Carmen

AU - Ouzari, Hadda

PY - 2012/12

Y1 - 2012/12

N2 - This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615.

AB - This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615.

KW - Antibiotic resistance

KW - Bacteriocins

KW - Enterococci

KW - MLST

KW - PFGE

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JO - Microbes and Environments

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IS - 4

ER -

Genotypic diversity, antibiotic resistance and bacteriocin production of enterococci isolated from rhizospheres

Abstract

Keywords

ASJC Scopus subject areas

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