Genomic analyses of a novel bioemulsifier-producing Psychrobacillus strain isolated from soil of King George Island, Antarctica

Mariana Barbalho Farias da Silva, Fabio Faria da Mota, Diogo Jurelevicius, Vasco Ariston de Carvalho Azevedo, Mateus Matiuzzi da Costa, Aristóteles Góes-Neto, Rommel Thiago Jucá Ramos, Siomar de Castro Soares, Alexandre S. Rosado, Lucy Seldin

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cold-adapted bacterial strains are potentially valuable for biotechnological applications involving the production of cold-active enzymes and bioproducts important to various industries. A psychrotolerant, aerobic, Gram-positive, endospore-forming, bioemulsifier-producing strain, named Val9, was isolated from Vale Ulman soil samples, King George Island, Antarctica and identified as a member of the genus Psychrobacillus. To better characterize this novel strain, its whole genome was sequenced revealing a size of 3,986,526 bp with a G + C content of 36.6%, and 4042 predicted coding DNA sequences (CDSs). Digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses between strain Val9 and the type strains of the seven Psychrobacillus species revealed that the highest values were observed with Psychrobacillus psychrodurans DSM11713T but below the conventional thresholds of 70% dDDH and 95% ANI for bacterial species assignment, suggesting that strain Val9 could represent a distinct species. As potential low-temperature adaptation strategies, genes encoding cold shock proteins, transporters for glycine-betaine, carnitine and choline, and enzymes acting against oxidative stress were found in Val9 genome. DEAD-box RNA helicases, important for cold and oxidative tolerance, and a two-component signal transduction system related to plasmatic membrane fluidity as well as biotechnologically important CDSs, related to levan production, were detected. The sacB gene encoding the enzyme levansucrase was exclusive for Val9 and it was not found in the other Psychrobacillus type strains. Altogether, the comparative genomic analyses presented here highlight important metabolic pathways and the biotechnological potential of this novel strain.
Original languageEnglish (US)
JournalPolar Biology
DOIs
StatePublished - Feb 25 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-04-26
Acknowledgements: Supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). Thanks are due to RECOM (“Rede de Ciências Ômicas”) for exchanging scientific knowledge among its participants, and to the reviewers (Dr. Carmen Rizzo, Dr. Annarita Poli, and Dr. Marco Fondi) for the improvement of the manuscript.

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences

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