Genome-wide identification and characterisation of exapted transposable elements in the large genome of sunflower (Helianthus annuus L.)

Maria Ventimiglia, Giovanni Marturano, Alberto Vangelisti, Gabriele Usai, Samuel Simoni, Andrea Cavallini, Tommaso Giordani, Lucia Natali, Andrea Zuccolo, Flavia Mascagni

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Transposable elements (TEs) are an important source of genome variability, playing many roles in the evolution of eukaryotic species. Besides well-known phenomena, TEs may undergo the exaptation process and generate the so-called exapted transposable element genes (ETEs). Here we present a genome-wide survey of ETEs in the large genome of sunflower (Helianthus annuus L.), in which the massive amount of TEs, provides a significant source for exaptation. A library of sunflower TEs was used to build TE-specific Hidden Markov Model profiles, to search for all available sunflower gene products. In doing so, 20,016 putative ETEs were identified and further investigated for the characteristics that distinguish TEs from genes, leading to the validation of 3,530 ETEs. The analysis of ETEs transcription patterns under different stress conditions showed a differential regulation triggered by treatments mimicking biotic and abiotic stress; furthermore, the distribution of functional domains of differentially regulated ETEs revealed a relevant presence of domains involved in many aspects of cellular functions. A comparative genomic investigation was performed including species representative of Asterids and appropriate outgroups: the bulk of ETEs resulted specific to the sunflower, while few ETEs presented orthologues in the genome of all analysed species, making the hypothesis of a conserved function. This study highlights the crucial role played by exaptation, actively contributing to species evolution.
Original languageEnglish (US)
JournalThe Plant Journal
DOIs
StatePublished - Dec 27 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-12-29
Acknowledgements: The authors acknowledge funding from the Department of Agriculture, Food, and Environment of the University of Pisa, Italy, Project “Plantomics”

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

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