Genome sequences of three
 Aegilops
 species of the section Sitopsis reveal phylogenetic relationships and provide resources for wheat improvement

Raz Avni; Thomas Lux; Anna Minz-Dub; Eitan Millet; Hanan Sela; Assaf Distelfeld; Jasline Deek; Guotai Yu; Burkhard Steuernagel; Curtis Pozniak; Jennifer Ens; Heidrun Gundlach; Klaus F. X. Mayer; Axel Himmelbach; Nils Stein; Martin Mascher; Manuel Spannagl; Brande B. H. Wulff; Amir Sharon

doi:10.1111/tpj.15664

Genome sequences of three Aegilops species of the section Sitopsis reveal phylogenetic relationships and provide resources for wheat improvement

Raz Avni, Thomas Lux, Anna Minz-Dub, Eitan Millet, Hanan Sela, Assaf Distelfeld, Jasline Deek, Guotai Yu, Burkhard Steuernagel, Curtis Pozniak, Jennifer Ens, Heidrun Gundlach, Klaus F. X. Mayer, Axel Himmelbach, Nils Stein, Martin Mascher, Manuel Spannagl, Brande B. H. Wulff, Amir Sharon

King Abdullah University of Science and Technology

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Aegilops is a close relative of wheat (Triticum spp.), and Aegilops species in the section Sitopsis represent a rich reservoir of genetic diversity for improvement of wheat. To understand their diversity and advance their utilization, we produced whole-genome assemblies of Ae. longissima and Ae. speltoides. Whole-genome comparative analysis, along with the recently sequenced Ae. sharonensis genome, showed that the Ae. longissima and Ae. sharonensis genomes are highly similar and most closely related to the wheat D subgenome. By contrast, the Ae. speltoides genome is more closely related to the B subgenome. Haplotype block analysis supported the idea that Ae. speltoides is the closest to the wheat B subgenome and highlighted variable and similar genomic regions between the three Aegilops species and wheat. Genome-wide analysis of nucleotide-binding leucine-rich repeat (NLR) genes revealed species-specific and lineage-specific NLR genes and variants, demonstrating the potential of Aegilops genomes for wheat improvement.

Original language	English (US)
Journal	The Plant Journal
DOIs	https://doi.org/10.1111/tpj.15664
State	Published - Jan 8 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-01-18
Acknowledgements: We are grateful to the Harold and Adele Lieberman Germplasm Bank, Tel Aviv University, for making Ae. longissima, Ae. sharonensis, and WEW seeds available, and to the Lieberman Family and JNF Australia for the generous support of this project. We kindly acknowledge Manuela Knauft and Ines Walde for technical assistance on Hi-C library preparation and sequencing. We thank Prof. Moshe Feldman for helpful discussions on the section Sitopsis. We thank Anne Fiebig for sequence data submission. Funding: This work was also supported by Genome Canada, Genome Prairie, and the Saskatchewan Ministry of Agriculture (CJP, JE); the Israel Science Foundation (ISF grant 1137/17; AD); the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to BBHW (BBS/E/J/000PR9780).

ASJC Scopus subject areas

Plant Science
Cell Biology
Genetics

Access to Document

10.1111/tpj.15664

https://repository.kaust.edu.sa/bitstream/10754/674982/1/The%20Plant%20Journal%20-%202022%20-%20Avni%20-%20Genome%20sequences%20of%20three%20Aegilops%20species%20of%20the%20section%20Sitopsis%20reveal%20phylogenetic.pdf

Cite this

Avni, R., Lux, T., Minz-Dub, A., Millet, E., Sela, H., Distelfeld, A., Deek, J., Yu, G., Steuernagel, B., Pozniak, C., Ens, J., Gundlach, H., Mayer, K. F. X., Himmelbach, A., Stein, N., Mascher, M., Spannagl, M., Wulff, B. B. H., & Sharon, A. (2022). Genome sequences of three Aegilops species of the section Sitopsis reveal phylogenetic relationships and provide resources for wheat improvement. The Plant Journal. https://doi.org/10.1111/tpj.15664

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title = "Genome sequences of three Aegilops species of the section Sitopsis reveal phylogenetic relationships and provide resources for wheat improvement",

abstract = "Aegilops is a close relative of wheat (Triticum spp.), and Aegilops species in the section Sitopsis represent a rich reservoir of genetic diversity for improvement of wheat. To understand their diversity and advance their utilization, we produced whole-genome assemblies of Ae. longissima and Ae. speltoides. Whole-genome comparative analysis, along with the recently sequenced Ae. sharonensis genome, showed that the Ae. longissima and Ae. sharonensis genomes are highly similar and most closely related to the wheat D subgenome. By contrast, the Ae. speltoides genome is more closely related to the B subgenome. Haplotype block analysis supported the idea that Ae. speltoides is the closest to the wheat B subgenome and highlighted variable and similar genomic regions between the three Aegilops species and wheat. Genome-wide analysis of nucleotide-binding leucine-rich repeat (NLR) genes revealed species-specific and lineage-specific NLR genes and variants, demonstrating the potential of Aegilops genomes for wheat improvement.",

author = "Raz Avni and Thomas Lux and Anna Minz-Dub and Eitan Millet and Hanan Sela and Assaf Distelfeld and Jasline Deek and Guotai Yu and Burkhard Steuernagel and Curtis Pozniak and Jennifer Ens and Heidrun Gundlach and Mayer, {Klaus F. X.} and Axel Himmelbach and Nils Stein and Martin Mascher and Manuel Spannagl and Wulff, {Brande B. H.} and Amir Sharon",

note = "KAUST Repository Item: Exported on 2022-01-18 Acknowledgements: We are grateful to the Harold and Adele Lieberman Germplasm Bank, Tel Aviv University, for making Ae. longissima, Ae. sharonensis, and WEW seeds available, and to the Lieberman Family and JNF Australia for the generous support of this project. We kindly acknowledge Manuela Knauft and Ines Walde for technical assistance on Hi-C library preparation and sequencing. We thank Prof. Moshe Feldman for helpful discussions on the section Sitopsis. We thank Anne Fiebig for sequence data submission. Funding: This work was also supported by Genome Canada, Genome Prairie, and the Saskatchewan Ministry of Agriculture (CJP, JE); the Israel Science Foundation (ISF grant 1137/17; AD); the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to BBHW (BBS/E/J/000PR9780).",

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Avni, R, Lux, T, Minz-Dub, A, Millet, E, Sela, H, Distelfeld, A, Deek, J, Yu, G, Steuernagel, B, Pozniak, C, Ens, J, Gundlach, H, Mayer, KFX, Himmelbach, A, Stein, N, Mascher, M, Spannagl, M, Wulff, BBH & Sharon, A 2022, 'Genome sequences of three Aegilops species of the section Sitopsis reveal phylogenetic relationships and provide resources for wheat improvement', The Plant Journal. https://doi.org/10.1111/tpj.15664

TY - JOUR

T1 - Genome sequences of three Aegilops species of the section Sitopsis reveal phylogenetic relationships and provide resources for wheat improvement

AU - Avni, Raz

AU - Lux, Thomas

AU - Minz-Dub, Anna

AU - Millet, Eitan

AU - Sela, Hanan

AU - Distelfeld, Assaf

AU - Deek, Jasline

AU - Yu, Guotai

AU - Steuernagel, Burkhard

AU - Pozniak, Curtis

AU - Ens, Jennifer

AU - Gundlach, Heidrun

AU - Mayer, Klaus F. X.

AU - Himmelbach, Axel

AU - Stein, Nils

AU - Mascher, Martin

AU - Spannagl, Manuel

AU - Wulff, Brande B. H.

AU - Sharon, Amir

N1 - KAUST Repository Item: Exported on 2022-01-18 Acknowledgements: We are grateful to the Harold and Adele Lieberman Germplasm Bank, Tel Aviv University, for making Ae. longissima, Ae. sharonensis, and WEW seeds available, and to the Lieberman Family and JNF Australia for the generous support of this project. We kindly acknowledge Manuela Knauft and Ines Walde for technical assistance on Hi-C library preparation and sequencing. We thank Prof. Moshe Feldman for helpful discussions on the section Sitopsis. We thank Anne Fiebig for sequence data submission. Funding: This work was also supported by Genome Canada, Genome Prairie, and the Saskatchewan Ministry of Agriculture (CJP, JE); the Israel Science Foundation (ISF grant 1137/17; AD); the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to BBHW (BBS/E/J/000PR9780).

PY - 2022/1/8

Y1 - 2022/1/8

N2 - Aegilops is a close relative of wheat (Triticum spp.), and Aegilops species in the section Sitopsis represent a rich reservoir of genetic diversity for improvement of wheat. To understand their diversity and advance their utilization, we produced whole-genome assemblies of Ae. longissima and Ae. speltoides. Whole-genome comparative analysis, along with the recently sequenced Ae. sharonensis genome, showed that the Ae. longissima and Ae. sharonensis genomes are highly similar and most closely related to the wheat D subgenome. By contrast, the Ae. speltoides genome is more closely related to the B subgenome. Haplotype block analysis supported the idea that Ae. speltoides is the closest to the wheat B subgenome and highlighted variable and similar genomic regions between the three Aegilops species and wheat. Genome-wide analysis of nucleotide-binding leucine-rich repeat (NLR) genes revealed species-specific and lineage-specific NLR genes and variants, demonstrating the potential of Aegilops genomes for wheat improvement.

AB - Aegilops is a close relative of wheat (Triticum spp.), and Aegilops species in the section Sitopsis represent a rich reservoir of genetic diversity for improvement of wheat. To understand their diversity and advance their utilization, we produced whole-genome assemblies of Ae. longissima and Ae. speltoides. Whole-genome comparative analysis, along with the recently sequenced Ae. sharonensis genome, showed that the Ae. longissima and Ae. sharonensis genomes are highly similar and most closely related to the wheat D subgenome. By contrast, the Ae. speltoides genome is more closely related to the B subgenome. Haplotype block analysis supported the idea that Ae. speltoides is the closest to the wheat B subgenome and highlighted variable and similar genomic regions between the three Aegilops species and wheat. Genome-wide analysis of nucleotide-binding leucine-rich repeat (NLR) genes revealed species-specific and lineage-specific NLR genes and variants, demonstrating the potential of Aegilops genomes for wheat improvement.

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SN - 0960-7412

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