Genome interplay in the grain transcriptome of hexaploid bread wheat

Matthias Pfeifer, Karl G. Kugler, Simen R. Sandve, Bujie Zhan, Heidi Rudi, Torgeir R. Hvidsten, Klaus F.X. Mayer, Odd Arne Olsen, Jane Rogers, Jaroslav Doležel, Curtis Pozniak, Kellye Eversole, Catherine Feuillet, Bikram Gill, Bernd Friebe, Adam J. Lukaszewski, Pierre Sourdille, Takashi R. Endo, Marie Kubaláková, Jarmila ČíhalíkováZdeňka Dubská, Jan Vrána, Romana Šperková, Hana Šimková, Melanie Febrer, Leah Clissold, Kirsten McLay, Kuldeep Singh, Parveen Chhuneja, Nagendra K. Singh, Jitendra Khurana, Eduard Akhunov, Frédéric Choulet, Adriana Alberti, Valérie Barbe, Patrick Wincker, Hiroyuki Kanamori, Fuminori Kobayashi, Takeshi Itoh, Takashi Matsumoto, Hiroaki Sakai, Tsuyoshi Tanaka, Jianzhong Wu, Yasunari Ogihara, Hirokazu Handa, P. Ron Maclachlan, Andrew Sharpe, Darrin Klassen, David Edwards, Jacqueline Batley, Simen Rød Sandve, Sigbjørn Lien, Burkhard Steuernagel, Brande Wulff, Mario Caccamo, Sarah Ayling, Ricardo H. Ramirez-Gonzalez, Bernardo J. Clavijo, Jonathan Wright, Manuel Spannagl, Mihaela M. Martis, Martin Mascher, Jarrod Chapman, Jesse A. Poland, Uwe Scholz, Kerrie Barry, Robbie Waugh, Daniel S. Rokhsar, Gary J. Muehlbauer, Nils Stein, Heidrun Gundlach, Matthias Zytnicki, Véronique Jamilloux, Hadi Quesneville, Thomas Wicker, Primetta Faccioli, Moreno Colaiacovo, Antonio Michele Stanca, Hikmet Budak, Luigi Cattivelli, Natasha Glover, Lise Pingault, Etienne Paux, Sapna Sharma, Rudi Appels, Matthew Bellgard, Brett Chapman, Thomas Nussbaumer, Kai Christian Bader, Hélène Rimbert, Shichen Wang, Ron Knox, Andrzej Kilian, Michael Alaux, Françoise Alfama, Loïc Couderc, Nicolas Guilhot, Claire Viseux, Mikaël Loaec, Beat Keller, Sebastien Praud

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226 Scopus citations

Abstract

Allohexaploid bread wheat (Triticum aestivum L) provides approximately 20% of calories consumed by humans. Lack of genome sequence for the three homeologous and highly similar bread wheat genomes (A, B, and D) has impeded expression analysis of the grain transcriptome. We used previously unknown genome information to analyze the cell type-specific expression of homeologous genes in the developing wheat grain and identified distinct co-expression clusters reflecting the spatiotemporal progression during endosperm development. We observed no global but cell type- and stage-dependent genome dominance, organization of the wheat genome into transcriptionally active chromosomal regions, and asymmetric expression in gene families related to baking quality. Our findings give insight into the transcriptional dynamics and genome interplay among individual grain cell types in a polyploid cereal genome.
Original languageEnglish (US)
JournalScience
Volume345
Issue number6194
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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