Genetic improvement of tomato by targeted control of fruit softening

Selman Uluisik; Natalie H Chapman; Rebecca Smith; Mervin Poole; Gary Adams; Richard B Gillis; Tabot M.D. Besong; Judith Sheldon; Suzy Stiegelmeyer; Laura Perez; Nurul Samsulrizal; Duoduo Wang; Ian D Fisk; Ni Yang; Charles Baxter; Daniel Rickett; Rupert Fray; Barbara Blanco-Ulate; Ann L T Powell; Stephen E Harding; Jim Craigon; Jocelyn K C Rose; Eric A Fich; Li Sun; David S Domozych; Paul D Fraser; Gregory A Tucker; Don Grierson; Graham B Seymour

doi:10.1038/nbt.3602

Genetic improvement of tomato by targeted control of fruit softening

Selman Uluisik, Natalie H Chapman, Rebecca Smith, Mervin Poole, Gary Adams, Richard B Gillis, Tabot M.D. Besong, Judith Sheldon, Suzy Stiegelmeyer, Laura Perez, Nurul Samsulrizal, Duoduo Wang, Ian D Fisk, Ni Yang, Charles Baxter, Daniel Rickett, Rupert Fray, Barbara Blanco-Ulate, Ann L T Powell, Stephen E HardingJim Craigon, Jocelyn K C Rose, Eric A Fich, Li Sun, David S Domozych, Paul D Fraser, Gregory A Tucker, Don Grierson, Graham B Seymour

Physical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

271 Scopus citations

Abstract

Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' that slow ripening and improve shelf life, but adversely affect flavor and color. We report substantial, targeted control of tomato softening, without affecting other aspects of ripening, by silencing a gene encoding a pectate lyase. © 2016 Nature America, Inc. All rights reserved.

Original language	English (US)
Pages (from-to)	950-952
Number of pages	3
Journal	Nature Biotechnology
Volume	34
Issue number	9
DOIs	https://doi.org/10.1038/nbt.3602
State	Published - Jul 25 2016

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: S.U. was funded by Ministry of Education of the Turkish Republic. The work was partly funded by BBSRC and Syngenta Seeds Ltd. through BBSRC 'stand-alone LINK' grants to P.D.F. and G.B.S. (BB/J015598/1 and BB/J016071/1). As part of the BBSRC grant, Syngenta staff (J.S., S.S., C.B. and D.R.) provided support with generating the transgenic plants, the bioinformatics analysis, the microscopy and writing the paper. G.B.S. and P.D.F. acknowledge support from EU project FP6 EUSOL and the European Cooperation in Science and Technology (COST) Action FA1106. D.D. was funded by US National Science Foundation grants NSF-MRI 1337280 and NSF-MRI 0922805. B.B.-U. and A.L.T.P. were funded by US National Science Foundation grants IOS 0957264 and IOS 0544504. J.R. was funded by a grant (IOS-1339287) from the Plant Genome Research Program of the US National Science Foundation. We acknowledge Syngenta Crop Protection, Research Triangle Park, North Carolina, USA, M. Franco for cDNA library preparation and J. Ni for RNASeq quality checks, read alignment and gene counting. We acknowledge J. Jones, V. Nekrasov and S. Kamoun, T.S.L. and The Gatsby Charitable Foundation for provision of the CRISPR-Cas 9 vectors. We also thank M. Bennett and J. Labavitch for useful discussions. COS488 was kindly provided by J. Mravec and W.G.T. Willats of the Department of Plant and Environmental Sciences of the University of Copenhagen.

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Uluisik, S., Chapman, N. H., Smith, R., Poole, M., Adams, G., Gillis, R. B., Besong, T. M. D., Sheldon, J., Stiegelmeyer, S., Perez, L., Samsulrizal, N., Wang, D., Fisk, I. D., Yang, N., Baxter, C., Rickett, D., Fray, R., Blanco-Ulate, B., Powell, A. L. T., ... Seymour, G. B. (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34(9), 950-952. https://doi.org/10.1038/nbt.3602

@article{e1e48f31c8704db997aec5cd5a3825e5,

title = "Genetic improvement of tomato by targeted control of fruit softening",

abstract = "Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' that slow ripening and improve shelf life, but adversely affect flavor and color. We report substantial, targeted control of tomato softening, without affecting other aspects of ripening, by silencing a gene encoding a pectate lyase. {\textcopyright} 2016 Nature America, Inc. All rights reserved.",

author = "Selman Uluisik and Chapman, {Natalie H} and Rebecca Smith and Mervin Poole and Gary Adams and Gillis, {Richard B} and Besong, {Tabot M.D.} and Judith Sheldon and Suzy Stiegelmeyer and Laura Perez and Nurul Samsulrizal and Duoduo Wang and Fisk, {Ian D} and Ni Yang and Charles Baxter and Daniel Rickett and Rupert Fray and Barbara Blanco-Ulate and Powell, {Ann L T} and Harding, {Stephen E} and Jim Craigon and Rose, {Jocelyn K C} and Fich, {Eric A} and Li Sun and Domozych, {David S} and Fraser, {Paul D} and Tucker, {Gregory A} and Don Grierson and Seymour, {Graham B}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: S.U. was funded by Ministry of Education of the Turkish Republic. The work was partly funded by BBSRC and Syngenta Seeds Ltd. through BBSRC 'stand-alone LINK' grants to P.D.F. and G.B.S. (BB/J015598/1 and BB/J016071/1). As part of the BBSRC grant, Syngenta staff (J.S., S.S., C.B. and D.R.) provided support with generating the transgenic plants, the bioinformatics analysis, the microscopy and writing the paper. G.B.S. and P.D.F. acknowledge support from EU project FP6 EUSOL and the European Cooperation in Science and Technology (COST) Action FA1106. D.D. was funded by US National Science Foundation grants NSF-MRI 1337280 and NSF-MRI 0922805. B.B.-U. and A.L.T.P. were funded by US National Science Foundation grants IOS 0957264 and IOS 0544504. J.R. was funded by a grant (IOS-1339287) from the Plant Genome Research Program of the US National Science Foundation. We acknowledge Syngenta Crop Protection, Research Triangle Park, North Carolina, USA, M. Franco for cDNA library preparation and J. Ni for RNASeq quality checks, read alignment and gene counting. We acknowledge J. Jones, V. Nekrasov and S. Kamoun, T.S.L. and The Gatsby Charitable Foundation for provision of the CRISPR-Cas 9 vectors. We also thank M. Bennett and J. Labavitch for useful discussions. COS488 was kindly provided by J. Mravec and W.G.T. Willats of the Department of Plant and Environmental Sciences of the University of Copenhagen.",

year = "2016",

month = jul,

day = "25",

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Uluisik, S, Chapman, NH, Smith, R, Poole, M, Adams, G, Gillis, RB, Besong, TMD, Sheldon, J, Stiegelmeyer, S, Perez, L, Samsulrizal, N, Wang, D, Fisk, ID, Yang, N, Baxter, C, Rickett, D, Fray, R, Blanco-Ulate, B, Powell, ALT, Harding, SE, Craigon, J, Rose, JKC, Fich, EA, Sun, L, Domozych, DS, Fraser, PD, Tucker, GA, Grierson, D & Seymour, GB 2016, 'Genetic improvement of tomato by targeted control of fruit softening', Nature Biotechnology, vol. 34, no. 9, pp. 950-952. https://doi.org/10.1038/nbt.3602

TY - JOUR

T1 - Genetic improvement of tomato by targeted control of fruit softening

AU - Uluisik, Selman

AU - Chapman, Natalie H

AU - Smith, Rebecca

AU - Poole, Mervin

AU - Adams, Gary

AU - Gillis, Richard B

AU - Besong, Tabot M.D.

AU - Sheldon, Judith

AU - Stiegelmeyer, Suzy

AU - Perez, Laura

AU - Samsulrizal, Nurul

AU - Wang, Duoduo

AU - Fisk, Ian D

AU - Yang, Ni

AU - Baxter, Charles

AU - Rickett, Daniel

AU - Fray, Rupert

AU - Blanco-Ulate, Barbara

AU - Powell, Ann L T

AU - Harding, Stephen E

AU - Craigon, Jim

AU - Rose, Jocelyn K C

AU - Fich, Eric A

AU - Sun, Li

AU - Domozych, David S

AU - Fraser, Paul D

AU - Tucker, Gregory A

AU - Grierson, Don

AU - Seymour, Graham B

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: S.U. was funded by Ministry of Education of the Turkish Republic. The work was partly funded by BBSRC and Syngenta Seeds Ltd. through BBSRC 'stand-alone LINK' grants to P.D.F. and G.B.S. (BB/J015598/1 and BB/J016071/1). As part of the BBSRC grant, Syngenta staff (J.S., S.S., C.B. and D.R.) provided support with generating the transgenic plants, the bioinformatics analysis, the microscopy and writing the paper. G.B.S. and P.D.F. acknowledge support from EU project FP6 EUSOL and the European Cooperation in Science and Technology (COST) Action FA1106. D.D. was funded by US National Science Foundation grants NSF-MRI 1337280 and NSF-MRI 0922805. B.B.-U. and A.L.T.P. were funded by US National Science Foundation grants IOS 0957264 and IOS 0544504. J.R. was funded by a grant (IOS-1339287) from the Plant Genome Research Program of the US National Science Foundation. We acknowledge Syngenta Crop Protection, Research Triangle Park, North Carolina, USA, M. Franco for cDNA library preparation and J. Ni for RNASeq quality checks, read alignment and gene counting. We acknowledge J. Jones, V. Nekrasov and S. Kamoun, T.S.L. and The Gatsby Charitable Foundation for provision of the CRISPR-Cas 9 vectors. We also thank M. Bennett and J. Labavitch for useful discussions. COS488 was kindly provided by J. Mravec and W.G.T. Willats of the Department of Plant and Environmental Sciences of the University of Copenhagen.

PY - 2016/7/25

Y1 - 2016/7/25

N2 - Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' that slow ripening and improve shelf life, but adversely affect flavor and color. We report substantial, targeted control of tomato softening, without affecting other aspects of ripening, by silencing a gene encoding a pectate lyase. © 2016 Nature America, Inc. All rights reserved.

AB - Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' that slow ripening and improve shelf life, but adversely affect flavor and color. We report substantial, targeted control of tomato softening, without affecting other aspects of ripening, by silencing a gene encoding a pectate lyase. © 2016 Nature America, Inc. All rights reserved.

UR - http://hdl.handle.net/10754/621550

UR - http://www.nature.com/articles/nbt.3602

UR - http://www.scopus.com/inward/record.url?scp=84986592867&partnerID=8YFLogxK

U2 - 10.1038/nbt.3602

DO - 10.1038/nbt.3602

M3 - Article

SN - 1087-0156

VL - 34

SP - 950

EP - 952

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 9

ER -

Genetic improvement of tomato by targeted control of fruit softening

Abstract

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