Genetic architecture and QTL selection response for Kernza perennial grain domestication traits

Jared Crain, Steve Larson, Kevin Dorn, Lee DeHaan, Jesse Poland

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Perennial grains have the potential to provide food for humans and decrease the negative impacts of annual agriculture. Intermediate wheatgrass (IWG, Thinopyrum intermedium, Kernza®) is a promising perennial grain candidate that The Land Institute has been breeding since 2003. We evaluated four consecutive breeding cycles of IWG from 2016 to 2020 with each cycle containing approximately 1100 unique genets. Using genotyping-by-sequencing markers, quantitative trait loci (QTL) were mapped for 34 different traits using genome-wide association analysis. Combining data across cycles and years, we found 93 marker-trait associations for 16 different traits, with each association explaining 0.8–5.2% of the observed phenotypic variance. Across the four cycles, only three QTL showed an FST differentiation > 0.15 with two corresponding to a decrease in floret shattering. Additionally, one marker associated with brittle rachis was 216 bp from an ortholog of the btr2 gene. Power analysis and quantitative genetic theory were used to estimate the effective number of QTL, which ranged from a minimum of 33 up to 558 QTL for individual traits. This study suggests that key agronomic and domestication traits are under polygenic control and that molecular methods like genomic selection are needed to accelerate domestication and improvement of this new crop.
Original languageEnglish (US)
JournalTheoretical and Applied Genetics
DOIs
StatePublished - Jun 28 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-06-30
Acknowledgements: This work was funded in part by the Perennial Agriculture Project in conjunction with the Malone Family Land Preservation Foundation and The Land Institute. Shuangye Wu provided vital laboratory support, and Marty Christians provided invaluable field assistance. The Thinopyrum intermedium Genome Sequencing Consortium provided prepublication access to the IWG genome sequence. Computational work was completed on the Beocat Research Cluster at Kansas State University, which is funded in part by NSF grants CNS-1006860, EPS-1006860, and EPS-0919443. Contribution no. 22-046-J from the Kansas Agricultural Experiment Station. The authors appreciate the constructive comments of two anonymous reviewers which improved the manuscript.
This work was funded by The Perennial Agriculture Project, in conjunction with the Malone Family Land Preservation Foundation and The Land Institute.

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Biotechnology

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