Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system

Robin Canac; Amandine Caillaud; Bastien Cimarosti; Aurore Girardeau; Hanan Hamamy; Bruno Reversade; Carine Bonnard; Zeina R. Al Sayed; Laurent David; Jeremie Poschmann; Patricia Lemarchand; Guillaume Lamirault; Nathalie Gaborit

doi:10.1016/j.scr.2021.102627

Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system

Robin Canac, Amandine Caillaud, Bastien Cimarosti, Aurore Girardeau, Hanan Hamamy, Bruno Reversade, Carine Bonnard, Zeina R. Al Sayed, Laurent David, Jeremie Poschmann, Patricia Lemarchand, Guillaume Lamirault, Nathalie Gaborit

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3 Scopus citations

Abstract

Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines: two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).

Original language	English (US)
Journal	Stem Cell Research
Volume	58
DOIs	https://doi.org/10.1016/j.scr.2021.102627
State	Published - Jan 1 2022
Externally published	Yes

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10.1016/j.scr.2021.102627

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Canac, R., Caillaud, A., Cimarosti, B., Girardeau, A., Hamamy, H., Reversade, B., Bonnard, C., Al Sayed, Z. R., David, L., Poschmann, J., Lemarchand, P., Lamirault, G., & Gaborit, N. (2022). Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system. Stem Cell Research, 58. https://doi.org/10.1016/j.scr.2021.102627

@article{daffffba74564edd845e4f069d4f900b,

title = "Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system",

abstract = "Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines: two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).",

author = "Robin Canac and Amandine Caillaud and Bastien Cimarosti and Aurore Girardeau and Hanan Hamamy and Bruno Reversade and Carine Bonnard and {Al Sayed}, {Zeina R.} and Laurent David and Jeremie Poschmann and Patricia Lemarchand and Guillaume Lamirault and Nathalie Gaborit",

note = "Generated from Scopus record by KAUST IRTS on 2023-02-15",

year = "2022",

month = jan,

day = "1",

doi = "10.1016/j.scr.2021.102627",

language = "English (US)",

volume = "58",

journal = "Stem Cell Research",

issn = "1873-5061",

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Canac, R, Caillaud, A, Cimarosti, B, Girardeau, A, Hamamy, H, Reversade, B, Bonnard, C, Al Sayed, ZR, David, L, Poschmann, J, Lemarchand, P, Lamirault, G & Gaborit, N 2022, 'Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system', Stem Cell Research, vol. 58. https://doi.org/10.1016/j.scr.2021.102627

TY - JOUR

T1 - Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system

AU - Canac, Robin

AU - Caillaud, Amandine

AU - Cimarosti, Bastien

AU - Girardeau, Aurore

AU - Hamamy, Hanan

AU - Reversade, Bruno

AU - Bonnard, Carine

AU - Al Sayed, Zeina R.

AU - David, Laurent

AU - Poschmann, Jeremie

AU - Lemarchand, Patricia

AU - Lamirault, Guillaume

AU - Gaborit, Nathalie

N1 - Generated from Scopus record by KAUST IRTS on 2023-02-15

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines: two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).

AB - Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines: two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).

UR - https://linkinghub.elsevier.com/retrieve/pii/S1873506121004748

UR - http://www.scopus.com/inward/record.url?scp=85121303211&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2021.102627

DO - 10.1016/j.scr.2021.102627

M3 - Article

C2 - 34929443

SN - 1873-5061

VL - 58

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system

Abstract

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