Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology.

Maryam Alowaysi; Mohammad Al-Shehri; Moayad Baadhaim; Hajar AlZahrani; Doaa Aboalola; Mustafa Daghestani; Heba Hashem; Rawan Aljahdali; Rayan Salem; Adel Alharbi; Mohammed Muharraq; Khaled Alghamdi; Fawaz Alsobiy; Asima Zia; Robert Lehmann; Jesper Tegner; Khaled Alsayegh

doi:10.1016/j.scr.2023.103158

Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology.

Maryam Alowaysi, Mohammad Al-Shehri, Moayad Baadhaim, Hajar AlZahrani, Doaa Aboalola, Mustafa Daghestani, Heba Hashem, Rawan Aljahdali, Rayan Salem, Adel Alharbi, Mohammed Muharraq, Khaled Alghamdi, Fawaz Alsobiy, Asima Zia, Robert Lehmann, Jesper Tegner, Khaled Alsayegh

Research output: Contribution to journal › Article › peer-review

Abstract

Myoglobin (MB) is a cytoplasmic hemoprotein that is predominantly expressed in the heart and oxidative myofibers of skeletal muscle. It has been demonstrated that MB binds to oxygen and promotes its diffusion for energy production in the mitochondria. Recently, MB was found to be expressed in different forms of malignant tumors and cancer cell lines. Further studies using gene disruption technology will enhance the understanding of MB’s role in human cardiovascular biology and cancers. Here, we describe the generation of a homozygous MB knockout in human embryonic stem cells (hESC-MB−/−) via CRISPR/Cas9 to study MB function in human biology and diseases.

Original language	English (US)
Pages (from-to)	103158
Journal	Stem Cell Research
Volume	71
DOIs	https://doi.org/10.1016/j.scr.2023.103158
State	Published - Jul 3 2023

Bibliographical note

KAUST Repository Item: Exported on 2023-07-10
Acknowledgements: This study was funded by King Abdullah International Medical Research Center (KAIMRC) research grant number NRJ21J/170/06.

ASJC Scopus subject areas

Cell Biology
Developmental Biology
General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.scr.2023.103158

https://repository.kaust.edu.sa/bitstream/10754/692857/1/1-s2.0-S1873506123001447-main.pdf

Cite this

Alowaysi, M., Al-Shehri, M., Baadhaim, M., AlZahrani, H., Aboalola, D., Daghestani, M., Hashem, H., Aljahdali, R., Salem, R., Alharbi, A., Muharraq, M., Alghamdi, K., Alsobiy, F., Zia, A., Lehmann, R., Tegner, J., & Alsayegh, K. (2023). Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology. Stem Cell Research, 71, 103158. https://doi.org/10.1016/j.scr.2023.103158

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title = "Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology.",

abstract = "Myoglobin (MB) is a cytoplasmic hemoprotein that is predominantly expressed in the heart and oxidative myofibers of skeletal muscle. It has been demonstrated that MB binds to oxygen and promotes its diffusion for energy production in the mitochondria. Recently, MB was found to be expressed in different forms of malignant tumors and cancer cell lines. Further studies using gene disruption technology will enhance the understanding of MB{\textquoteright}s role in human cardiovascular biology and cancers. Here, we describe the generation of a homozygous MB knockout in human embryonic stem cells (hESC-MB−/−) via CRISPR/Cas9 to study MB function in human biology and diseases.",

author = "Maryam Alowaysi and Mohammad Al-Shehri and Moayad Baadhaim and Hajar AlZahrani and Doaa Aboalola and Mustafa Daghestani and Heba Hashem and Rawan Aljahdali and Rayan Salem and Adel Alharbi and Mohammed Muharraq and Khaled Alghamdi and Fawaz Alsobiy and Asima Zia and Robert Lehmann and Jesper Tegner and Khaled Alsayegh",

note = "KAUST Repository Item: Exported on 2023-07-10 Acknowledgements: This study was funded by King Abdullah International Medical Research Center (KAIMRC) research grant number NRJ21J/170/06.",

year = "2023",

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doi = "10.1016/j.scr.2023.103158",

language = "English (US)",

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Alowaysi, M, Al-Shehri, M, Baadhaim, M, AlZahrani, H, Aboalola, D, Daghestani, M, Hashem, H, Aljahdali, R, Salem, R, Alharbi, A, Muharraq, M, Alghamdi, K, Alsobiy, F, Zia, A , Lehmann, R , Tegner, J & Alsayegh, K 2023, 'Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology.', Stem Cell Research, vol. 71, pp. 103158. https://doi.org/10.1016/j.scr.2023.103158

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T1 - Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology.

AU - Alowaysi, Maryam

AU - Al-Shehri, Mohammad

AU - Baadhaim, Moayad

AU - AlZahrani, Hajar

AU - Aboalola, Doaa

AU - Daghestani, Mustafa

AU - Hashem, Heba

AU - Aljahdali, Rawan

AU - Salem, Rayan

AU - Alharbi, Adel

AU - Muharraq, Mohammed

AU - Alghamdi, Khaled

AU - Alsobiy, Fawaz

AU - Zia, Asima

AU - Lehmann, Robert

AU - Tegner, Jesper

AU - Alsayegh, Khaled

N1 - KAUST Repository Item: Exported on 2023-07-10 Acknowledgements: This study was funded by King Abdullah International Medical Research Center (KAIMRC) research grant number NRJ21J/170/06.

PY - 2023/7/3

Y1 - 2023/7/3

N2 - Myoglobin (MB) is a cytoplasmic hemoprotein that is predominantly expressed in the heart and oxidative myofibers of skeletal muscle. It has been demonstrated that MB binds to oxygen and promotes its diffusion for energy production in the mitochondria. Recently, MB was found to be expressed in different forms of malignant tumors and cancer cell lines. Further studies using gene disruption technology will enhance the understanding of MB’s role in human cardiovascular biology and cancers. Here, we describe the generation of a homozygous MB knockout in human embryonic stem cells (hESC-MB−/−) via CRISPR/Cas9 to study MB function in human biology and diseases.

AB - Myoglobin (MB) is a cytoplasmic hemoprotein that is predominantly expressed in the heart and oxidative myofibers of skeletal muscle. It has been demonstrated that MB binds to oxygen and promotes its diffusion for energy production in the mitochondria. Recently, MB was found to be expressed in different forms of malignant tumors and cancer cell lines. Further studies using gene disruption technology will enhance the understanding of MB’s role in human cardiovascular biology and cancers. Here, we describe the generation of a homozygous MB knockout in human embryonic stem cells (hESC-MB−/−) via CRISPR/Cas9 to study MB function in human biology and diseases.

UR - http://hdl.handle.net/10754/692857

UR - https://linkinghub.elsevier.com/retrieve/pii/S1873506123001447

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SP - 103158

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology.

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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